Abstract | OBJECTIVES: METHODS: Dahl salt-sensitive rats were fed a normal (0.5% NaCl) or high- salt diet (4% NaCl), or high- salt diet and pyrrolidine dithiocarbamat (150 mg/kg in drinking water), an inhibitor of NF kappaB activation, for 6 weeks. RESULTS: Hypertensive Dahl salt-sensitive rats manifested impaired endothelium-dependent relaxation to acetylcholine, aortic hypertrophy (35%), increased plasma C-reactive protein (25%), vascular superoxide (O2) production (148%), and expression of monocyte chemoattractant protein-1, tumor necrosis factor alpha, phospho-I kappaB alpha, and phospho-(Ser536)-p65NF kappaB. Pyrrolidine dithiocarbamat significantly improved endothelium-dependent relaxation, reduced vascular O2, and normalized aortic hypertrophy and systemic and local inflammation, despite only mildly reducing blood pressure. Hypertensive Dahl salt-sensitive rats also manifested impaired insulin-mediated vasorelaxation and Akt/ endothelial nitric oxide synthase phosphorylation and decreased insulin sensitivity by hyperinsulinemic-euglycemic clamp ( glucose infusion rate, -32%). Pyrrolidine dithiocarbamat significantly improved insulin-mediated vascular relaxation and Akt/ endothelial nitric oxide synthase phosphorylation as well as insulin sensitivity. CONCLUSION:
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Authors | Ming-Sheng Zhou, Ivonne Hernandez Schulman, Leopoldo Raij |
Journal | Journal of hypertension
(J Hypertens)
Vol. 28
Issue 3
Pg. 527-35
(Mar 2010)
ISSN: 1473-5598 [Electronic] England |
PMID | 19898250
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Chemokine CCL2
- Insulin
- NF-kappa B
- Tumor Necrosis Factor-alpha
- Sodium Chloride
- C-Reactive Protein
- Nitric Oxide Synthase Type III
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Blood Pressure
- Body Weight
- C-Reactive Protein
(metabolism)
- Chemokine CCL2
(metabolism)
- Endothelium, Vascular
(metabolism, physiopathology)
- Hypertension
(metabolism, physiopathology)
- Insulin
(physiology)
- Insulin Resistance
- Male
- NF-kappa B
(metabolism)
- Nitric Oxide Synthase Type III
(metabolism)
- Phosphorylation
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Rats, Inbred Dahl
- Rats, Sprague-Dawley
- Sodium Chloride
- Tumor Necrosis Factor-alpha
(metabolism)
- Vasculitis
(metabolism, physiopathology)
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