Sevoflurane postconditioning protects chronically-infarcted rat hearts against ischemia-reperfusion injury by activation of pro-survival kinases and inhibition of mitochondrial permeability transition pore opening upon reperfusion.

We evaluated the cardioprotection against myocardial ischemia-reperfusion injury induced by sevoflurane postconditioning (SpostC) in chronically-infarcted rat hearts, and investigated the roles of phosphoinositide 3-kinase (PI3K)-protein kinase B/Akt (PKB/Akt), mitogen-activated extracellular regulated kinase 1/2 (MEK 1/2)-extracellular regulated kinase 1/2 (ERK 1/2), and mitochondrial permeability transition pore (mPTP). Left anterior descending (LAD) coronary artery was ligated to induce myocardial infarction in rats. Six weeks later, chronically-infarcted hearts were isolated and subjected to 30 min of global ischemia, followed by 1 h of reperfusion with Krebs-Henseleit (K-H) buffer. SpostC was administered by perfusing the hearts with K-H buffer saturated with 3% sevoflurane during the first 15 min of reperfusion. To evaluate the role of PI3K-PKB/Akt and MEK 1/2-ERK 1/2 in SpostC, PI3K inhibitor LY294002 (15 microM) and MEK 1/2 inhibitor PD98059 (20 microM) were administered alone or together with sevoflurane during the first 15 min of reperfusion. We found that exposure of 3% sevoflurane during early reperfusion significantly improved functional recovery (improved left ventricular developed pressure (LVDP), +/-dp/dt, CF, HR and reduced left ventricular end-diastolic pressure (LVEDP)), decreased myocardial infarct size and reduced LDH and CK-MB release, when compared with unprotected hearts. However, these protective effects were abolished in the presence of either LY294002 or PD98059, which was accompanied by the prevention of PKB/Akt and ERK 1/2 phosphorylation, and reduction of myocardial nicotinamide adenine dinucleotide (NAD+) content. These findings suggest that sevoflurane postconditioning protects chronically-infarcted rat hearts against ischemia-reperfusion injury by inhibiting mPTP opening via recruitment of PKB/Akt and ERK 1/2.
AuthorsYuntai Yao, Lihuan Li, Libing Li, Changqing Gao, Chunxia Shi
JournalBiological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 32 Issue 11 Pg. 1854-61 (Nov 2009) ISSN: 1347-5215 [Electronic] Japan
PMID19881297 (Publication Type: Journal Article)
Chemical References
  • Anesthetics, Inhalation
  • Methyl Ethers
  • sevoflurane
  • Protein Kinases
  • Anesthetics, Inhalation (pharmacology)
  • Animals
  • Blotting, Western
  • Enzyme Activation
  • Male
  • Methyl Ethers (pharmacology)
  • Mitochondria, Heart (drug effects)
  • Myocardial Infarction (complications)
  • Permeability
  • Protein Kinases (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury (prevention & control)

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