Abstract | BACKGROUND: OBJECTIVE: This study aimed at defining the T-cell immunogenicity of food AGEs by using ovalbumin (OVA) as a model allergen. METHODS: AGE-OVA was prepared by means of thermal processing of OVA in the presence of glucose. Activation of OVA-specific CD4(+) T cells by AGE-OVA was evaluated in cocultures with bone marrow-derived murine myeloid dendritic cells (mDCs) as antigen-presenting cells. The uptake mechanisms of mDCs for AGE-OVA were investigated by using inhibitors of putative cell-surface receptors for AGEs, as well as mDCs deficient for these receptors. RESULTS: Compared with the controls (native OVA and OVA thermally processed without glucose), AGE-OVA enhanced the activation of OVA-specific CD4(+) T cells on coculture with mDCs, indicating that the glycation of OVA enhanced the T-cell immunogenicity of the allergen. The mDC uptake of AGE-OVA was significantly higher than that of the controls. We identified scavenger receptor class A type I and II (SR-AI/II) as a mediator of the AGE-OVA uptake, whereas the receptor for AGEs and galectin-3 were not responsible. Importantly, the activation of OVA-specific CD4(+) T cells by AGE-OVA was attenuated on coculture with SR-AI/II-deficient mDCs. CONCLUSION: SR-AI/II targets AGE-OVA to the MHC class II loading pathway in mDCs, leading to an enhanced CD4(+) T-cell activation. The Maillard reaction might thus play an important role in the T-cell immunogenicity of food allergens.
|
Authors | Anne Ilchmann, Sven Burgdorf, Stephan Scheurer, Zoe Waibler, Ryoji Nagai, Anne Wellner, Yasuhiko Yamamoto, Hiroshi Yamamoto, Thomas Henle, Christian Kurts, Ulrich Kalinke, Stefan Vieths, Masako Toda |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 125
Issue 1
Pg. 175-83.e1-11
(Jan 2010)
ISSN: 1097-6825 [Electronic] United States |
PMID | 19864011
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved. |
Chemical References |
- Allergens
- Glycation End Products, Advanced
- Scavenger Receptors, Class A
- Ovalbumin
|
Topics |
- Allergens
(immunology, metabolism)
- Animals
- Antigen Presentation
- Bone Marrow Cells
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Coculture Techniques
- Dendritic Cells
(cytology, immunology)
- Food Hypersensitivity
(immunology, physiopathology)
- Glycation End Products, Advanced
(immunology, metabolism)
- Lymphocyte Activation
- Maillard Reaction
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Ovalbumin
(immunology, metabolism)
- Scavenger Receptors, Class A
(metabolism)
- T-Lymphocytes
(cytology, immunology)
|