Abstract | CONCLUSIONS: Human papilloma virus (HPV) was associated with sinonasal inverted papilloma (SIP) in 14/20 (70%) patients with a prevalence of HPV 6/11; alterations of the cell cycle proteins were statistically significant. OBJECTIVES: MATERIALS AND METHODS: Twenty SIPs were evaluated for p53, p16(INK4a), pRb, p21(WAF1), p27(Kip1), cyclin D1 and Ki-67 expression by immunohistochemistry. HPV was investigated by polymerase chain reaction (PCR). RESULTS: HPV DNA was detected in 14/20 patients with inverted papillomas (IPs) (70%). The majority of tumours showed strong p16, p21, p27, pRb and cyclin D1 staining and little or no p53 expression. Tumours harbouring dysplasia were significantly more likely to be p53-positive and exhibit up-regulated p21 and p27, and showed altered intensity and distribution of reactive cells into and through the epithelium. Dysplastic epithelium was strongly reactive for p16 and the MIB 1 labelling index was almost 20%. These findings were associated with expression of p53 in the same zones. Comparing the p53 reactivity with the presence of HPV DNA, SIPs were stratified as follows: HPV + p53-, 12 (63.15%); HPV + p53+, 2 (10.52%); HPV - p53+, 3 (15.78%) and HPV - p53-, 2 (10.52%). Statistical analysis showed that HPV presence correlated with p53-positive immunostaining (p=0.045).
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Authors | Giuseppe Altavilla, Alberto Staffieri, Graziella Busatto, Alessandra Canesso, Luciano Giacomelli, Gino Marioni |
Journal | Acta oto-laryngologica
(Acta Otolaryngol)
Vol. 129
Issue 11
Pg. 1242-9
(Nov 2009)
ISSN: 1651-2251 [Electronic] England |
PMID | 19863319
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCND1 protein, human
- CDKN1A protein, human
- CDKN1B protein, human
- Cyclin-Dependent Kinase Inhibitor p16
- Cyclin-Dependent Kinase Inhibitor p21
- DNA Probes, HPV
- Intracellular Signaling Peptides and Proteins
- Ki-67 Antigen
- PRB1 protein, human
- Salivary Proline-Rich Proteins
- Tumor Suppressor Protein p53
- Cyclin D1
- Cyclin-Dependent Kinase Inhibitor p27
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Topics |
- Cyclin D1
(genetics)
- Cyclin-Dependent Kinase Inhibitor p16
(genetics)
- Cyclin-Dependent Kinase Inhibitor p21
(genetics)
- Cyclin-Dependent Kinase Inhibitor p27
- DNA Probes, HPV
- Gene Expression Regulation
(genetics)
- Gene Expression Regulation, Viral
(genetics)
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics)
- Ki-67 Antigen
(genetics)
- Nose Neoplasms
(genetics, pathology)
- Papilloma, Inverted
(genetics, pathology)
- Papillomaviridae
(genetics)
- Papillomavirus Infections
(genetics, pathology)
- Paranasal Sinus Neoplasms
(genetics, pathology)
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
(genetics)
- Salivary Proline-Rich Proteins
(genetics)
- Tumor Suppressor Protein p53
(genetics)
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