Expression of p53, p16INK4A, pRb, p21WAF1/CIP1, p27KIP1, cyclin D1, Ki-67 and HPV DNA in sinonasal endophytic Schneiderian (inverted) papilloma.

Abstract

CONCLUSIONS:

Human papilloma virus (HPV) was associated with sinonasal inverted papilloma (SIP) in 14/20 (70%) patients with a prevalence of HPV 6/11; alterations of the cell cycle proteins were statistically significant.

OBJECTIVES:

We investigated SIPs relationships between HPV infection and aberrant expression of cell cycle proteins.

MATERIALS AND METHODS:

Twenty SIPs were evaluated for p53, p16(INK4a), pRb, p21(WAF1), p27(Kip1), cyclin D1 and Ki-67 expression by immunohistochemistry. HPV was investigated by polymerase chain reaction (PCR).

RESULTS:

HPV DNA was detected in 14/20 patients with inverted papillomas (IPs) (70%). The majority of tumours showed strong p16, p21, p27, pRb and cyclin D1 staining and little or no p53 expression. Tumours harbouring dysplasia were significantly more likely to be p53-positive and exhibit up-regulated p21 and p27, and showed altered intensity and distribution of reactive cells into and through the epithelium. Dysplastic epithelium was strongly reactive for p16 and the MIB 1 labelling index was almost 20%. These findings were associated with expression of p53 in the same zones. Comparing the p53 reactivity with the presence of HPV DNA, SIPs were stratified as follows: HPV + p53-, 12 (63.15%); HPV + p53+, 2 (10.52%); HPV - p53+, 3 (15.78%) and HPV - p53-, 2 (10.52%). Statistical analysis showed that HPV presence correlated with p53-positive immunostaining (p=0.045).

• Authors: Giuseppe Altavilla, Alberto Staffieri, Graziella Busatto, Alessandra Canesso, Luciano Giacomelli, Gino Marioni
• Journal: Acta oto-laryngologica (Acta Otolaryngol) Vol. 129 Issue 11 Pg. 1242-9 (Nov 2009) ISSN: 1651-2251 [Electronic] England
• PMID: 19863319 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCND1 protein, human
• CDKN1A protein, human
• CDKN1B protein, human
• Cyclin-Dependent Kinase Inhibitor p16
• Cyclin-Dependent Kinase Inhibitor p21
• DNA Probes, HPV
• Intracellular Signaling Peptides and Proteins
• Ki-67 Antigen
• PRB1 protein, human
• Salivary Proline-Rich Proteins
• Tumor Suppressor Protein p53
• Cyclin D1
• Cyclin-Dependent Kinase Inhibitor p27

Topics

• Cyclin D1 (genetics)
• Cyclin-Dependent Kinase Inhibitor p16 (genetics)
• Cyclin-Dependent Kinase Inhibitor p21 (genetics)
• Cyclin-Dependent Kinase Inhibitor p27
• DNA Probes, HPV
• Gene Expression Regulation (genetics)
• Gene Expression Regulation, Viral (genetics)
• Humans
• Intracellular Signaling Peptides and Proteins (genetics)
• Ki-67 Antigen (genetics)
• Nose Neoplasms (genetics, pathology)
• Papilloma, Inverted (genetics, pathology)
• Papillomaviridae (genetics)
• Papillomavirus Infections (genetics, pathology)
• Paranasal Sinus Neoplasms (genetics, pathology)
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length (genetics)
• Salivary Proline-Rich Proteins (genetics)
• Tumor Suppressor Protein p53 (genetics)