Expression of gamma-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116.

Abstract

AIM:

To investigate the expression pattern of gamma-synuclein in colorectal cancer (CRC) tissues, and to study the effects of gamma-synuclein on CRC cell line HCT116 biological features in vitro.

METHODS:

The expression pattern of gamma-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between gamma-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA (siRNA) targeting gamma-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro.

RESULTS:

The expression of gamma-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the gamma-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of gamma-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells.

CONCLUSION:

Increased expression of gamma-synuclein in CRC tissues and the biological effects of reduced gamma-synuclein expression on HCT116 cells suggest that gamma-synuclein may play a positive role in the progression of CRC.

• Authors: Qing Ye, Bo Feng, Yuan-Fei Peng, Xue-Hua Chen, Qu Cai, Bei-Qin Yu, Liang-Hui Li, Ming-Yuan Qiu, Bing-Ya Liu, Min-Hua Zheng
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 15 Issue 40 Pg. 5035-43 (Oct 28 2009) ISSN: 2219-2840 [Electronic] United States
• PMID: 19859996 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• RNA, Small Interfering
• gamma-Synuclein

Topics

• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Colorectal Neoplasms (metabolism)
• Disease Progression
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry (methods)
• Neoplasm Invasiveness
• RNA, Messenger (metabolism)
• RNA, Small Interfering (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Time Factors
• gamma-Synuclein (biosynthesis)