Abstract | AIM: METHODS: The expression pattern of gamma-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between gamma-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA ( siRNA) targeting gamma-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS: The expression of gamma-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the gamma-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of gamma-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells. CONCLUSION:
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Authors | Qing Ye, Bo Feng, Yuan-Fei Peng, Xue-Hua Chen, Qu Cai, Bei-Qin Yu, Liang-Hui Li, Ming-Yuan Qiu, Bing-Ya Liu, Min-Hua Zheng |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 15
Issue 40
Pg. 5035-43
(Oct 28 2009)
ISSN: 2219-2840 [Electronic] United States |
PMID | 19859996
(Publication Type: Journal Article)
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Chemical References |
- RNA, Messenger
- RNA, Small Interfering
- gamma-Synuclein
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Topics |
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Colorectal Neoplasms
(metabolism)
- Disease Progression
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
(methods)
- Neoplasm Invasiveness
- RNA, Messenger
(metabolism)
- RNA, Small Interfering
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
- gamma-Synuclein
(biosynthesis)
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