HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Western blot analysis for the detection of serum antibodies recognizing linear Aquaporin-4 epitopes in patients with Neuromyelitis Optica.

Abstract
The current study presents a western blot assay showing good sensitivity (81%) and specificity (97%) for detecting serum antibodies to Aquaporin-4 (AQP4) in patients with Neuromyelitis Optica (NMO). By using western blot, we were able, for the first time, to distinguish serum immunoreactivity against either of the two AQP4 isoforms, showing that antibodies recognizing the linear AQP4-M1 isoform are specific for NMO. Moreover, the high sensitivity and specificity of the western blot assay in detecting serum anti-AQP4 antibodies in NMO patients suggest that such auto-antibodies may be of linear nature, thus recognizing epitopes that are displayed in denatured protein.
AuthorsFabiana Marnetto, Bianca Hellias, Letizia Granieri, Jessica Frau, Agata Katia Patanella, Petra Nytrova, Arianna Sala, Marco Capobianco, Francesca Gilli, Antonio Bertolotto
JournalJournal of neuroimmunology (J Neuroimmunol) Vol. 217 Issue 1-2 Pg. 74-9 (Dec 10 2009) ISSN: 1872-8421 [Electronic] Netherlands
PMID19850359 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • AQP4 protein, human
  • Antibodies
  • Aquaporin 4
  • Epitopes
  • Protein Isoforms
Topics
  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies (blood)
  • Aquaporin 4 (immunology)
  • Blotting, Western (methods)
  • Child
  • Epitope Mapping (methods)
  • Epitopes (immunology)
  • Female
  • Fluorescent Antibody Technique (methods)
  • Humans
  • Immunoprecipitation (methods)
  • Male
  • Mice
  • Middle Aged
  • Multiple Sclerosis (blood)
  • Neuromyelitis Optica (blood)
  • Protein Array Analysis (methods)
  • Protein Isoforms (immunology)
  • Sensitivity and Specificity
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: