Abstract |
In shrimp, small GTPases in the Ras superfamily can regulate hemolytic phagocytosis against WSSV infection. However, the ADP ribosylation factors (Arfs), also belonging to the regulatory GTP-binding proteins and playing a central role in membrane trafficking, have not been reported yet in shrimp and their relationship with WSSV infection is completely unknown to date. Here, a novel class II Arf (designated as MjArf4) was cloned and characterized from the shrimp Marsupenaeus japonicus. Like other Arfs, MjArf4 contains an N-terminal myristoylated site, a p loop, switch regions, as well as an interswitch region. In High Five cells, when MjArf4 was in its GDP-bound form, it dispersed into the whole cell, whereas in the GTP-bound form it promoted formation of a punctuate Golgi-like structure, indicating that the MjArf4 distribution was dependent on its GDP/ GTP binding. After challenge with WSSV, the mRNA level of MjArf4 was up-regulated significantly as WSSV propagated. Thus, a member of the Arf family was characterized for the first time in shrimp and found to be involved in WSSV infection.
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Authors | Mingchang Zhang, Jinyou Ma, Kaiyu Lei, Xun Xu |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 28
Issue 1
Pg. 128-33
(Jan 2010)
ISSN: 1095-9947 [Electronic] England |
PMID | 19837172
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- ADP-Ribosylation Factors
(biosynthesis, genetics, physiology)
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Virus Infections
(immunology)
- Molecular Sequence Data
- Penaeidae
(enzymology, genetics, immunology, virology)
- Reverse Transcriptase Polymerase Chain Reaction
- White spot syndrome virus 1
(immunology)
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