In vitro cytotoxic effects of benzalkonium chloride on adenovirus.

Abstract

OBJECTIVE:

To investigate the in vitro effects of benzalkonium chloride (BAK) at various concentrations and dosing schedules on adenoviral infection.

METHODS:

Adenovirus was placed in Refeed media and at 30 min after infection, various concentrations and doses of BAK were placed into the 1 mL solution. All assays were incubated at 37 degrees C. Dosing of BAK was performed for 3 days and qualitative viral immunofluorescence was compared with that of controls.

RESULTS:

BAK at multiple concentrations lysed the epithelial cell lines to varying degrees as did viral infection. Further, adenoviral infection was halted with many concentrations as well.

CONCLUSIONS:

Adenoviral infection was inhibited in vitro by different concentrations of BAK.

CLINICAL RELEVANCE:

Zymar solution which contains BAK could potentially have a role in the treatment of adenoviral ocular infection in humans.

• Authors: Douglas R Lazzaro, Khaled Abulawi, Mohammedyusuf E Hajee
• Journal: Eye & contact lens (Eye Contact Lens) Vol. 35 Issue 6 Pg. 329-32 (Nov 2009) ISSN: 1542-233X [Electronic] United States
• PMID: 19826252 (Publication Type: Journal Article)

Chemical References

• Benzalkonium Compounds
• Preservatives, Pharmaceutical

Topics

• Adenoviridae (drug effects)
• Adenoviridae Infections (virology)
• Benzalkonium Compounds (administration & dosage, toxicity)
• Cell Line
• Dose-Response Relationship, Drug
• Drug Administration Schedule
• Epithelial Cells (virology)
• Fluorescent Antibody Technique
• Microbial Sensitivity Tests
• Preservatives, Pharmaceutical (administration & dosage, toxicity)