Guilandina bonduc L. possesses antioxidant activity and precludes Ferric Nitrilotriacetate (Fe-NTA) induced renal toxicity and tumor promotion response.

Abstract

Guilandina bonduc Linn. (Fabaceae), a prickly shrub that grows abundantly in tropical and subtropical regions of Southeast Asia, possesses an extensive medicinal value. The present communication reports ethanolic extract of G. bonduc fruit pods to possess potent antioxidant activity and significantly protect against Ferric Nitrilotriacetate (Fe-NTA)-induced renal oxi-dative stress, nephrotoxicity, and tumor promotion response. Intraperitoneal administration of Fe-NTA (9 mg Fe/Kg body weight) to Swiss albino mice depleted renal antioxidant armory (glutathione content and activities of antioxidant enzymes), and caused oxidative stress, induced inflammation (inducing the expression of inducible nitric oxide synthase and cyclooxygenase-2 and increasing the secretion of proinflammatory cytokines). It also induced hyperproliferation in kidney-enhancing ornithine decarboxylase (ODC) activity and [3H]-thymidine incorporation into DNA. Histopathological changes and levels of serum creatinine (SCr) and blood urea nitrogen (BUN) suggested Fe-NTA to afflict substantial damage to the kidney. Prophylactic treatment of mice with G. bonduc extract (100-200 mg /Kg body weight) for seven days not only restored renal antioxidant armory close to normal, but also significantly precluded Fe-NTA associated inflammation and hyperproliferation. Furthermore, pretreatment with extract markedly prevented renal oxidative damage and histopathological alterations, and restored the levels of renal damage markers close to control values. The data obtained in the present study indicate G. bonduc fruit pods to possess a potent antioxidant activity and avert chemically inflicted renal oxidative damage and tumor promotion response.

• Authors: Gurpreet Kaur, Irshad A Lone, Mohammad Athar, M Sarwar Alam
• Journal: Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer (J Environ Pathol Toxicol Oncol) Vol. 28 Issue 2 Pg. 163-75 ( 2009) ISSN: 2162-6537 [Electronic] United States
• PMID: 19817703 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Carcinogens
• Ferric Compounds
• Plant Extracts
• Ornithine Decarboxylase
• Nitrilotriacetic Acid
• ferric nitrilotriacetate

Topics

• Animals
• Antioxidants (pharmacology)
• Blotting, Western
• Carcinogens (toxicity)
• Chromatography, High Pressure Liquid
• DNA Replication
• Fabaceae (chemistry)
• Female
• Ferric Compounds (toxicity)
• Kidney (drug effects)
• Mice
• Neoplasms, Experimental (chemically induced, prevention & control)
• Nitrilotriacetic Acid (analogs & derivatives, toxicity)
• Ornithine Decarboxylase (metabolism)
• Oxidative Stress
• Plant Extracts (pharmacology)