In vivo dilatation of the ductus arteriosus induced by furosemide in the rat.

Abstract

Furosemide increases prostaglandin production and may be associated with patent ductus arteriosus (PDA). We aimed to clarify the in vivo ductus-dilating effects of furosemide in neonatal rats. Near-term rat pups delivered by a cesarean section were housed at 33 degrees C. After a rapid whole-body freezing, the DA diameter was measured using a microscope and a micrometer. Pregnant rats (gestational day 21) were s.c. injected with furosemide 4 h before delivery, and the neonatal DA was examined 0, 15, 30, 60, and 120 min after birth. Furosemide was also s.c. injected into 60-min-old rats and the DA diameter was examined 30, 60, and 120 min later. The control rats showed a rapid postnatal DA constriction (diameter: 0.80 and 0.08 mm at 0 and 60 min after birth, respectively). Prenatally administered furosemide delayed postnatal DA closure (0.36 mm at 60 min after birth). Furosemide injection in 60-min-old rats dilated the constricted DA at 60 min (0.25 versus 0.02 mm in the controls). Indomethacin inhibited furosemide-induced DA dilatation. Furosemide delays DA closure and dilates the constricted DA in neonatal rats. If furosemide has similar effects in human preterm neonates, caution may be warranted in its use in the treatment of infants with PDA.

• Authors: Katsuaki Toyoshima, Kazuo Momma, Toshio Nakanishi
• Journal: Pediatric research (Pediatr Res) Vol. 67 Issue 2 Pg. 173-6 (Feb 2010) ISSN: 1530-0447 [Electronic] United States
• PMID: 19809378 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclooxygenase Inhibitors
• Prostaglandins E
• Furosemide
• Indomethacin

Topics

• Animals
• Animals, Newborn
• Cesarean Section
• Cyclooxygenase Inhibitors (pharmacology)
• Dilatation, Pathologic
• Disease Models, Animal
• Ductus Arteriosus (drug effects, metabolism, pathology)
• Ductus Arteriosus, Patent (chemically induced, metabolism, pathology, prevention & control)
• Female
• Furosemide (administration & dosage)
• Gestational Age
• Indomethacin (pharmacology)
• Injections, Subcutaneous
• Pregnancy
• Prostaglandins E (metabolism)
• Rats
• Rats, Wistar
• Time Factors