Abstract | BACKGROUND: METHODS: PBV allergen threshold basophil sensitivity, CD-sens, was performed on cells from nine patients diagnosed as having had adverse reactions to PBV. The mechanisms of the CD-sens were studied by immunological and immuno-chemical methods. RESULTS: Five of the nine patients had a positive CD-sens to PBV which was completely eliminated by washing the cells in phosphate buffered saline before allergen challenge. However, the positive CD-sens was completely reconstituted by incubating the cells in plasma or serum of that patient or the other PBV-anaphylactic patients for 15 min at room temperature. In some patients the factor mediating CD-sens was completely or partially destroyed by heating at +56 degrees C for 30 min or being exposed to the low pH used for elution from anti-Ig columns. A 1000-fold excess of monoclonal IgE blocked the reconstitution by approximately 50%. CONCLUSION:
Anaphylactic reactions to PBV are mediated by IgE antibodies giving a classical CD-sens reaction. However, the allergenic configuration seems to constitute a structure completely dependent on PBV, as a hapten, linked to a, so far, unknown carrier that seems to be unique for patients having experienced a PBV-induced reaction. Further studies are needed to characterize the postulated carrier.
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Authors | S G O Johansson, A Nopp, H Oman, P Stahl-Skov, A S Hunting, A B Guttormsen |
Journal | Allergy
(Allergy)
Vol. 65
Issue 1
Pg. 124-9
(Jan 2010)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 19804438
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Coloring Agents
- Rosaniline Dyes
- Immunoglobulin E
- sulfan blue
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Topics |
- Anaphylaxis
(chemically induced, immunology)
- Basophils
(immunology)
- Coloring Agents
(adverse effects)
- Humans
- Immunoglobulin E
(immunology)
- Rosaniline Dyes
(adverse effects, immunology)
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