Comparison of 2.5% sodium hypochlorite and 2% chlorhexidine gel on oral bacterial lipopolysaccharide reduction from primarily infected root canals.

Abstract

INTRODUCTION:

This clinical study was conducted to compare the efficacy of chemomechanical preparation with 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) gel on eliminating oral bacterial lipopolysaccharide (LPS) in teeth with pulp necrosis and apical periodontitis.

METHODS:

Fifty-four root canals were selected. Samples were collect before (s1) and after chemomechanical preparation (s2). Teeth were randomly divided into groups: GI, 2.5% NaOCl (n = 27) and GII, CHX gel (n = 27). Limulus amebocyte lysate assay was used to quantify endotoxins.

RESULTS:

Endotoxin was present in 100% of the samples investigated, with a median value of 272 endotoxin units (EU)/mL (GI) and of 152.46 EU/mL (GII). As a result of chemomechanical preparation, LPS content was reduced to a median value of 86 EU/mL (GI) and 85 EU/mL (GII). Higher percentage value of endotoxin reduction was found in GI (P < .05).

CONCLUSIONS:

The 2.5% NaOCl and 2% CHX gel were not effective in eliminating endotoxin from the primarily infected root canals.

• Authors: Brenda P F A Gomes, Frederico C Martinho, Morgana E Vianna
• Journal: Journal of endodontics (J Endod) Vol. 35 Issue 10 Pg. 1350-3 (Oct 2009) ISSN: 1878-3554 [Electronic] United States
• PMID: 19801228 (Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents, Local
• Chromogenic Compounds
• Endotoxins
• Lipopolysaccharides
• Root Canal Irrigants
• Sodium Hypochlorite
• Chlorhexidine

Topics

• Adolescent
• Adult
• Anti-Infective Agents, Local (therapeutic use)
• Chlorhexidine (therapeutic use)
• Chromogenic Compounds
• Dental Pulp Cavity (drug effects, microbiology)
• Dental Pulp Necrosis (microbiology, therapy)
• Endotoxins (analysis)
• Humans
• Lipopolysaccharides (analysis)
• Middle Aged
• Periapical Periodontitis (microbiology, therapy)
• Root Canal Irrigants (therapeutic use)
• Root Canal Preparation (methods)
• Sodium Hypochlorite (therapeutic use)
• Treatment Outcome
• Young Adult