Role of hydrogen sulfide in hepatic ischemia-reperfusion-induced injury in rats.

Abstract

Hydrogen sulfide (H2S) displays anti-inflammatory and cytoprotective activities as evidenced by the inhibition of myocardial ischemia-reperfusion injury and production of lipid peroxidation. H2S also exerts many physiological or pathological effects on livers. Therefore, we designed the present study to investigate the roles of H2S in hepatic ischemia-reperfusion (HIR)-induced injury in rats by measuring H2S levels, H2S synthesizing activity, and cystathionine gamma-lyase (CSE) messenger RNA (mRNA) expression. We also applied DL-propargyl glycine (PAG) and sodium hydrosulfide (NaHS) to investigate their effects on the severity of liver injury induced by HIR. The levels of H2S, H2S production activity, and CSE mRNA expression in livers were increased by HIR. Administration of NaHS significantly attenuated the severity of liver injury and inhibited the production of lipid peroxidation, serum inflammatory factors [including nitric oxide, tumor necrosis factor alpha (TNF-alpha), interleukin 10, and intercellular cell adhesion molecule 1], cell apoptosis, and apoptosis-related proteins (including caspase-3, Fas, Fas ligand, and TNF-alpha), which were caused or elevated by HIR, whereas PAG aggravated them. However, NaHS or PAG did not show significant effects on the activation of caspase-9, which was also increased by HIR. Although further investigation is required, this study may indicate that H2S plays a protective role in HIR-induced injury.

• Authors: Kai Kang, Mingyan Zhao, Hongchi Jiang, Gang Tan, Shangha Pan, Xueying Sun
• Journal: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society (Liver Transpl) Vol. 15 Issue 10 Pg. 1306-14 (Oct 2009) ISSN: 1527-6473 [Electronic] United States
• PMID: 19790158 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 AASLD

Chemical References

• Anti-Inflammatory Agents
• RNA, Messenger
• Cystathionine gamma-Lyase
• Hydrogen Sulfide

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Blotting, Western
• Cystathionine gamma-Lyase (metabolism)
• Hydrogen Sulfide (metabolism)
• Inflammation
• Lipid Peroxidation
• Liver (injuries)
• Male
• Models, Biological
• RNA, Messenger (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (diagnosis, etiology)
• Reverse Transcriptase Polymerase Chain Reaction