High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary.

Abstract

N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes beta1-6 branching of N-acetylglucosamine (GlcNAc) on asparagine-linked oligosaccharides of cell proteins. The present study aimed to investigate GnT-V expression and its prognostic significance in epithelial ovarian cancer. GnT-V expression was studied by immunohistochemistry in 83 surgically resected ovarian cancers, and the staining intensity was evaluated. High GnT-V expression in cancer cells was found in 17 (20.5%) of the 83 cases, and was positively correlated with early FIGO staging. In the histological type, mucinous adenocarcinoma showed significantly strong immunostaining compared to the non-mucinous type (P<0.001). In 36 mucinous tumors, the GnT-V immunostaining score was significantly higher in cancer than in benign and borderline tumors (P<0.001). NOM-1, a human ovarian mucinous adenocarcinoma cell line, expressed strong GnT-V protein and swainsonine treatment suppressed beta1-6GlcNAc branching and reduced migration ability significantly (P<0.001). These results suggested that GnT-V might be involved in the malignant potential of mucinous ovarian cancer.

• Authors: Noriko Takahashi, Eiko Yamamoto, Kazuhiko Ino, Eiji Miyoshi, Tetsuro Nagasaka, Hiroaki Kajiyama, Kiyosumi Shibata, Akihiro Nawa, Fumitaka Kikkawa
• Journal: Oncology reports (Oncol Rep) Vol. 22 Issue 5 Pg. 1027-32 (Nov 2009) ISSN: 1021-335X [Print] Greece
• PMID: 19787216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phytohemagglutinins
• N-Acetylglucosaminyltransferases
• alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Topics

• Adenocarcinoma, Clear Cell (enzymology, secondary, surgery)
• Adenocarcinoma, Mucinous (enzymology, secondary, surgery)
• Adult
• Aged
• Blotting, Western
• Cell Adhesion
• Cell Movement
• Cell Proliferation
• Cystadenocarcinoma, Serous (enzymology, secondary, surgery)
• Endometrial Neoplasms (enzymology, secondary, surgery)
• Female
• Humans
• Immunoenzyme Techniques
• Middle Aged
• N-Acetylglucosaminyltransferases (metabolism)
• Ovarian Neoplasms (enzymology, pathology, surgery)
• Phytohemagglutinins (metabolism)
• Prognosis
• Tumor Cells, Cultured