Abstract | BACKGROUND: In a study performed in 1983, 18 adult volunteers received oral challenge with the virulent human rotavirus strain D (G1P1A[8],NSP4[B]). To identify correlates of resistance to rotavirus infection, we analyzed levels of serum immunoglobulin (Ig) A and IgG antibodies to various rotaviral antigens in 16 of the 18 volunteers. METHODS: We used immunocytochemical assays that involved a total of 16 different recombinant baculoviruses, with each baculovirus expressing one of the following major serotype/genotype rotavirus proteins for the serologic assays: (1) viral protein (VP) 4 with P1A[8], P1B[4], P2A[6], P3[9], or P4[10] specificity; (2) VP7 with G1-G4 or G9 specificity; and (3) nonstructural viral protein (NSP) 4 with genotype A, B, C, or D specificity. RESULTS: The prechallenge titers of IgG antibody to VP7 types G1, G3, G4, and G9; VP4 types P1A[8], P1B[4], P2A[6], and P4[10]; and NSP4 type [A] in the group of noninfected volunteers (n = 11) were significantly higher than those in the group of infected volunteers (n = 5; of these 5 volunteers, 4 were symptomatically infected). Moreover, logistic regression analysis showed that resistance to rotavirus infection most closely correlated with higher prechallenge titers of IgG antibody to homotypic VP7 (G1) and VP4 (P1A[8]). CONCLUSIONS: These results suggest that protection against rotavirus infection and disease is primarily VP7/VP4 homotypic and, to a lesser degree, heterotypic.
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Authors | Lijuan Yuan, Shinjiro Honma, Inyoung Kim, Albert Z Kapikian, Yasutaka Hoshino |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 200
Issue 9
Pg. 1443-51
(Nov 01 2009)
ISSN: 1537-6613 [Electronic] United States |
PMID | 19785527
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Chemical References |
- Antigens, Viral
- Capsid Proteins
- Immunoglobulin A
- Immunoglobulin G
- VP4 protein, Rotavirus
- VP7 protein, Rotavirus
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Topics |
- Adolescent
- Adult
- Antibody Specificity
(immunology)
- Antigens, Viral
(classification, immunology)
- Capsid Proteins
(classification, immunology)
- Female
- Humans
- Immunoglobulin A
(immunology)
- Immunoglobulin G
(immunology)
- Male
- Rotavirus
(classification, immunology)
- Rotavirus Infections
(immunology)
- Young Adult
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