Type-specific antigens for serological discrimination of HTLV-I and HTLV-II infection.

Abstract

55 HTLV-I (human T-cell lymphotropic virus) and 45 HTLV-II carriers, confirmed by HTLV-type specific polymerase chain reaction (PCR), were distinguished by western blot assays with recombinant HTLV I or II envelope glycoproteins. Recombinant protein (RP) B1 contains aminoacids 166-201 from HTLV-I exterior glycoprotein gp46 and was reactive with HTLV-I samples only. RP-IIB, which contains aminoacids 96-235 from HTLV-II exterior glycoprotein gp52, was reactive with all HTLV-II samples. 39 patients (86.6%) had high reactivity by densitometry. Of 55 HTLV-I samples, 35 (65.5%) had antibody reactivity to RP-IIB, but only 1 (1.8%) had high reactivity by densitometry. RP B1 and IIB western blot assays may replace the PCR test in diagnosis of HTLV infection.

• Authors: Y M Chen, T H Lee, S Z Wiktor, G M Shaw, E L Murphy, W A Blattner, M Essex
• Journal: Lancet (London, England) (Lancet) Vol. 336 Issue 8724 Pg. 1153-5 (Nov 10 1990) ISSN: 0140-6736 [Print] England
• PMID: 1978028 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• HTLV-I Antigens
• HTLV-II Antigens
• Recombinant Proteins

Topics

• Blotting, Western
• Diagnosis, Differential
• HTLV-I Antigens (analysis, genetics)
• HTLV-I Infections (genetics, immunology)
• HTLV-II Antigens (analysis, genetics)
• HTLV-II Infections (genetics, immunology)
• Humans
• Polymerase Chain Reaction
• Recombinant Proteins