Abstract |
To investigate whether changes in systemic pH influence ketone body production or utilization, total ketone body (TK) kinetics were measured with [3-14C] acetoacetate and D-beta-[1,3-13C2]hydroxybutyrate tracers in overnight fasted subjects during metabolic alkalosis (NaHCO3 infusion) or acidosis [NH4Cl ingestion or arginine (Arg)-HCl infusion]. Somatostatin, with insulin, glucagon, and growth hormone replacement, was infused in all studies. Blood pH and HCO3- (mM) increased from baseline (0-30 min) to 180-210 min by 0.08 +/- 0.02 and 7 +/- 1 with NaHCO3 and decreased by 0.08 +/- 0.2 and 7 +/- 1 or 5 +/- 1 with NH4Cl or Arg-HCl (all P less than 0.005). Over this period blood TK (microM) differed between the NaHCO3 (+198 +/- 65) and both NH4Cl (-90 +/- 53) and Arg-HCl (-154 +/- 55) (P less than 0.05). These changes resulted from parallel alterations in TK production rate of appearance (Ra TK, mumol.kg-1.min-1), because changes from baseline in Ra 14C TK also differed between NaHCO3 (+1.9 +/- 0.8) and NH4Cl (-1.0 +/- 0.6) and Arg-HCl (-2.0 +/- 0.5) (P less than 0.05). Ra TK calculated with single- or dual-tracer techniques were similar. Blood free fatty acids (FFA) increased with NaHCO3, and FFA and glycerol decreased with NH4Cl and Arg-HCl, suggesting that FFA availability mediated the pH effects on hepatic ketogenesis. These results demonstrate that modest changes in systemic pH modify FFA availability and TK production rates.
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Authors | V L Hood, U Keller, M W Haymond, D Küry |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 259
Issue 3 Pt 1
Pg. E327-34
(Sep 1990)
ISSN: 0002-9513 [Print] United States |
PMID | 1975988
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bicarbonates
- Insulin
- Ketone Bodies
- Ammonium Chloride
- Somatostatin
- Sodium Bicarbonate
- Arginine
- Sodium
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Topics |
- Acid-Base Equilibrium
- Adult
- Ammonium Chloride
(pharmacology)
- Arginine
(pharmacology)
- Bicarbonates
(blood, pharmacology)
- Female
- Humans
- Hydrogen-Ion Concentration
- Insulin
(blood, pharmacology)
- Ketone Bodies
(blood, metabolism)
- Lipolysis
- Male
- Middle Aged
- Reference Values
- Sodium
(pharmacology)
- Sodium Bicarbonate
- Somatostatin
(pharmacology)
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