Abstract | BACKGROUND: An essential requirement related to treatment of dengue-infected patients is a rapid and accurate detection of dengue virus during febrile stage of the disease. OBJECTIVE: The study examined using direct immunofluorescence staining the presence of dengue viral antigen in peripheral blood mononuclear cells (PBMCs). METHODS: RESULTS:
Dengue viral antigens were found in most PBMC samples of dengue infected patients collected on the day before defervescence and continued until 2 to 3 days afterward. The number of dengue viral antigen positive PBMCs was highest in patients with dengue shock syndrome. Sensitivity and specificity of this method during the febrile stage was 93.8% (95% confidence interval: 88.8%-98.9%) and 100%, respectively. The positive and negative predictive values were 100% and 69.0%, respectively. CONCLUSION:
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Authors | Sirichan Chunhakan, Punnee Butthep, Sutee Yoksan, Kanchana Tangnararatchakit, Ampaiwan Chuansumrit |
Journal | The Pediatric infectious disease journal
(Pediatr Infect Dis J)
Vol. 28
Issue 12
Pg. 1085-8
(Dec 2009)
ISSN: 1532-0987 [Electronic] United States |
PMID | 19755928
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Antigens, Viral
(blood)
- Child
- Child, Preschool
- Dengue
(blood, diagnosis)
- Dengue Virus
(isolation & purification)
- Female
- Fluorescent Antibody Technique, Direct
- Humans
- Leukocytes, Mononuclear
(chemistry)
- Male
- Sensitivity and Specificity
- Young Adult
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