Abstract | OBJECTIVE: METHODS: RESULTS: The cytotoxin separated and purified from crude cobra venom caused intense cytotoxic effects to the BEL-7404 cells in vitro. The diameter of PLGA microspheres containing cobra venom cytotoxin was about (34.45+/-9.85)microm. Encapsulation rate was up to (78.13+/-8.92)%, and cumulative amount of cobra venom cytotoxin released from the PLGA microspheres in vitro during 30 days was up to 84.3%. After intratumoral injection, tumor volumes and weights in the cytotoxin-PLGA group were lower than those in the normal saline group, with a tumor growth inhibition rate of 52.36%. Observed under a light microscope, most tumor tissues were necrotic. No obvious morphological change could be seen on the liver, kidney and heart tissues. CONCLUSION: The above findings indicate that intratumoral injection of cytotoxin-PLGA microspheres has strong antitumor effect and can obviously lessen systemic toxicity, which may provide an effective and feasible method for hepatocellular carcinoma treatment.
|
Authors | Yan Wang, Li-wu Lin, Zhi-kui Chen, En-sheng Xue, Xiao-dong Lin, Li-Yun Yu, Zhen-hu Lin |
Journal | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
(Zhong Xi Yi Jie He Xue Bao)
Vol. 7
Issue 9
Pg. 836-41
(Sep 2009)
ISSN: 1672-1977 [Print] China |
PMID | 19747438
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Animals
- Carcinoma, Hepatocellular
(drug therapy)
- Elapid Venoms
(administration & dosage)
- Humans
- Injections, Intralesional
- Liver Neoplasms
(drug therapy)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Microspheres
- Neoplasm Transplantation
- Random Allocation
|