Mitigation of acute ultraviolet B radiation-mediated damages by baicalin in mouse skin.

Abstract

BACKGROUND:

Solar ultraviolet (UV) irradiation, in particular UVB with a wavelength range between 290 and 320 nm, induces different hazardous effects on the skin, including sunburn, photoaging and cancer. Protection against sun-induced damage is therefore a highly desirable goal. Chemoprevention is being investigated as a potential approach for the management of UV damages including skin cancer.

AIM:

In this study, to determine the relevance of our in vitro findings to in vivo situations, we assessed the effects of baicalin on UVB-mediated damages in mice skin.

METHODS:

Balb/C hairless mice were topically pretreated (24 h before UVB) or post-treated (5 min after UVB) with baicalin (1 mg/cm(2) skin area/mouse/100 microl acetone) and were exposed to UVB 24 h later (180 mJ/cm(2)). The animals were sacrificed 1 and 24 h after the UVB exposure. Skin edema, histopathology changes, hydrogen peroxide (H2O2) and cyclobutane pyrimidine dimers (CPDs)-positive cells were assessed to determine the UVB-induced photodamage.

RESULTS:

Our data demonstrated that a topical application of baicalin, either as a pretreatment or as a post-treatment, resulted in a significant decrease in UVB mediated increases in skin edema, skin hyperplasia and infiltration of leukocytes. Further, baicalin treatments (pre and post) also resulted in a significant decrease in UVB mediated (1) generation of H2O2 and (2) formation of DNA photolesions: CPDs.

CONCLUSION:

Based on these data, we suggest that baicalin could be developed as an agent for the management of conditions elicited by UV exposure including skin cancer.

• Authors: Bing-Rong Zhou, Bing-Jiang Lin, Song-Liang Jin, Dan Luo
• Journal: Photodermatology, photoimmunology & photomedicine (Photodermatol Photoimmunol Photomed) Vol. 25 Issue 5 Pg. 250-8 (Oct 2009) ISSN: 1600-0781 [Electronic] England
• PMID: 19747244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Pyrimidine Dimers
• baicalin
• Hydrogen Peroxide

Topics

• Animals
• DNA Damage
• Female
• Flavonoids (pharmacology)
• Hydrogen Peroxide (metabolism)
• Mice
• Mice, Inbred BALB C
• Pyrimidine Dimers (metabolism)
• Skin (drug effects, metabolism, radiation effects)
• Spectrophotometry, Ultraviolet
• Ultraviolet Rays