HLA-DQ beta-chain restriction fragment length polymorphism as a risk marker in type 1 (insulin-dependent) diabetes mellitus: a Finnish family study.

Abstract

Finnish Type 1 (insulin-dependent) diabetic families were analysed for HLA-DQ beta-chain polymorphism using a short intron-specific probe. A simple hybridization pattern was obtained in which all fragments were associated significantly with Type 1 diabetes. The simultaneous presence of two different risk markers, the allelic 12-kilobase and 4-kilobase fragments were strongly associated with Type 1 diabetes since 50% of the patients had this combination compared with only 2% of the control subjects. The cosegregated 7.5/3.0 kilobase fragments, which were associated with HLA-DR2 and DRw6 were not detected among the diabetic patients but were present in 48% of the control subjects. Our results provide further support for the location of susceptibility determining factors in the HLA-DQ gene area. The clear-cut, simple restriction fragment length polymorphism pattern obtained here, which bears a resemblance to a two allelic system, therefore makes this method applicable for estimating the risk of Type 1 diabetes at the population level.

• Authors: H Reijonen, J Ilonen, M Knip, B Michelsen, H K Akerblom
• Journal: Diabetologia (Diabetologia) Vol. 33 Issue 6 Pg. 357-62 (Jun 1990) ISSN: 0012-186X [Print] Germany
• PMID: 1974216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• HLA-DQ Antigens
• HLA-DQ beta-Chains
• HLA-DQbeta antigen
• HLA-DR Antigens
• Deoxyribonuclease BamHI

Topics

• Adolescent
• Alleles
• B-Lymphocytes (immunology)
• Biomarkers (analysis)
• Child
• Deoxyribonuclease BamHI
• Diabetes Mellitus, Type 1 (genetics, immunology)
• Finland
• Genotype
• HLA-DQ Antigens (genetics)
• HLA-DQ beta-Chains
• HLA-DR Antigens (genetics)
• Haplotypes
• Humans
• Polymorphism, Restriction Fragment Length
• Reference Values
• Risk Factors