Detection of bacteria by fluorescence in situ hybridization in culture-negative soft tissue filler lesions.

Abstract

BACKGROUND:

Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler.

OBJECTIVES:

Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization.

RESULTS:

Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates.

CONCLUSION:

This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment.

• Authors: Thomas Bjarnsholt, Tim Tolker-Nielsen, Michael Givskov, Martin Janssen, Lise Hanne Christensen
• Journal: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] (Dermatol Surg) Vol. 35 Suppl 2 Pg. 1620-4 (Oct 2009) ISSN: 1524-4725 [Electronic] United States
• PMID: 19709133 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acrylic Resins
• Anti-Bacterial Agents
• Biocompatible Materials
• Dermatologic Agents
• polyacrylamide gels

Topics

• Acrylic Resins (administration & dosage, adverse effects)
• Anti-Bacterial Agents (therapeutic use)
• Bacterial Infections (diagnosis, drug therapy)
• Biocompatible Materials (administration & dosage, adverse effects)
• Cosmetic Techniques (adverse effects)
• Dermatologic Agents (administration & dosage, adverse effects)
• Granuloma, Foreign-Body (diagnosis, drug therapy, microbiology)
• Humans
• In Situ Hybridization, Fluorescence
• Injections (adverse effects)
• Microbial Sensitivity Tests (methods)
• Rejuvenation
• Skin Aging
• Staphylococcal Infections (diagnosis)
• Staphylococcus (isolation & purification)
• Treatment Outcome