IL-6-transfected tumor cells modulate the status of CD8(+) and CD4(+) T cells to control tumor growth.

Abstract

IL-6 is a proinflammatory cytokine secreted by tumor cells and immune cells to affect the development of cancer. This study demonstrates the effects of tumor-derived IL-6 on the malignancy of tumor cells and tumor immunity. The tumor cell line, EG7, was transfected with a mammalian expression vector encoding the full length of murine IL-6 to mimic IL-6-secreting tumor cells. Two IL-6 transfectants with low and high IL-6 production were compared in vitro and in vivo. While the in vitro proliferation rates of both transfectants and the parental line were similar, high expression of IL-6 induced a significant reduction in tumor growth in vivo. Concomitantly, there was an increase in IFN-gamma positive tumor-infiltrating lymphocytes and a decrease in the suppressive CD4(+)CD25(+)FoxP3(+) population. These results demonstrate the direct effects of tumor-derived IL-6 on cancer development and the induction of tumor immunity.

• Authors: Chia-Ling Hsieh, Shih-Jen Liu, Chia-Rui Shen, Mei-Yu Chen, Shu-Ching Hsu, Jy-Ping Tsai, Yi-Shiuan Li, Ching-Jen Yang, Pele Chong, Hsin-Wei Chen
• Journal: Immunobiology (Immunobiology) Vol. 215 Issue 6 Pg. 486-91 (Jun 2010) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 19699006 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier GmbH. All rights reserved.

Chemical References

• Interleukin-6
• Interferon-gamma

Topics

• Animals
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Cell Line, Tumor
• Immunity, Cellular (genetics, immunology)
• Interferon-gamma (immunology)
• Interleukin-6 (genetics, immunology)
• Mice
• Mice, SCID
• Neoplasms, Experimental (immunology, therapy)
• Transfection