Abstract |
To diagnose Epstein-Barr virus (EBV)-associated diseases and to explore the pathogenesis of EBV infection, not only must the EBV load be measured, but EBV-infected cells must also be identified. We established a novel flow cytometric in situ hybridization assay to detect EBV(+) suspension cells using a peptide nucleic acid probe specific for EBV-encoded small RNA (EBER). By enhancing fluorescence and photostability, we successfully stained EBER and surface antigens on the same cells. In 3 patients with hydroa vacciniforme-like lymphoproliferative disease, we demonstrated that 1.7%-25.9% of peripheral lymphocytes were infected with EBV and specifically identified these lymphocytes as CD3(+)CD4(-)CD8(-) gammadelta T cell receptor-positive T cells. The results indicate that this novel and noninvasive assay is a direct and reliable method of characterizing EBV-infected lymphocytes that can be used not only to diagnose EBV infection but also to clarify the pathogenesis of EBV-associated diseases.
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Authors | Hiroshi Kimura, Kanae Miyake, Yohei Yamauchi, Kana Nishiyama, Seiko Iwata, Keiji Iwatsuki, Kensei Gotoh, Seiji Kojima, Yoshinori Ito, Yukihiro Nishiyama |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 200
Issue 7
Pg. 1078-87
(Oct 01 2009)
ISSN: 0022-1899 [Print] United States |
PMID | 19698076
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adolescent
- Cell Line, Tumor
- Child
- Flow Cytometry
- Herpesvirus 4, Human
(physiology)
- Humans
- Hydroa Vacciniforme
(virology)
- In Situ Hybridization, Fluorescence
- Lymphocyte Subsets
(cytology, virology)
- Lymphoproliferative Disorders
(etiology, virology)
- Male
- Sensitivity and Specificity
- Transplantation
(adverse effects)
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