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[A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever].

Abstract
We have previously evaluated a Semliki Forest virus (SFV) replicon vectored DNA vaccine (pSFV1CS2-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus (CSFV) in pigs. The results showed that the immunized pigs were protected from virulent challenge, but few pigs showed short-term fever and occasional pathological changes following virulent challenge. To enhance the immunogenecity of the vaccines, we tried a prime-boost vaccination strategy using a combination of prime with pSFV1CS2-E2 followed by boost with rAdV-E2. The results showed that all the immunized pigs developed high-level CSFV-specific antibodies following prime-boost immunization. When challenged with virulent CSFV, the immunized pigs (n = 5) from the heterologous boost group showed no clinical symptoms, and CSFV RNA was not detected following challenge, whereas one of five pigs from the homologous boost group developed short-term fever and CSFV RNA was detected. This demonstrates that the heterologous prime-boost vaccination regime has the potential to prevent against virulent challenge.
AuthorsYuan Sun, Dafei Liu, Yufei Wang, Na Li, Hongyu Li, Bingbing Liang, Huaji Qiu
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 25 Issue 5 Pg. 679-85 (May 2009) ISSN: 1000-3061 [Print] China
PMID19670635 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adenovirus E2 Proteins
  • Vaccines, DNA
  • Viral Envelope Proteins
  • Viral Vaccines
  • glycoprotein E2, classical swine fever virus
Topics
  • Adenoviridae (genetics, metabolism)
  • Adenovirus E2 Proteins (genetics, immunology)
  • Animals
  • Classical Swine Fever (immunology, prevention & control)
  • Classical Swine Fever Virus (genetics, immunology)
  • Genetic Vectors
  • Immunization, Secondary
  • Replicon (genetics)
  • Semliki forest virus (genetics, metabolism)
  • Swine
  • Vaccines, DNA (immunology)
  • Viral Envelope Proteins (genetics, metabolism)
  • Viral Vaccines (immunology)

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