Abstract |
We have previously evaluated a Semliki Forest virus (SFV) replicon vectored DNA vaccine (pSFV1CS2-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus (CSFV) in pigs. The results showed that the immunized pigs were protected from virulent challenge, but few pigs showed short-term fever and occasional pathological changes following virulent challenge. To enhance the immunogenecity of the vaccines, we tried a prime-boost vaccination strategy using a combination of prime with pSFV1CS2-E2 followed by boost with rAdV-E2. The results showed that all the immunized pigs developed high-level CSFV-specific antibodies following prime-boost immunization. When challenged with virulent CSFV, the immunized pigs (n = 5) from the heterologous boost group showed no clinical symptoms, and CSFV RNA was not detected following challenge, whereas one of five pigs from the homologous boost group developed short-term fever and CSFV RNA was detected. This demonstrates that the heterologous prime-boost vaccination regime has the potential to prevent against virulent challenge.
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Authors | Yuan Sun, Dafei Liu, Yufei Wang, Na Li, Hongyu Li, Bingbing Liang, Huaji Qiu |
Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology
(Sheng Wu Gong Cheng Xue Bao)
Vol. 25
Issue 5
Pg. 679-85
(May 2009)
ISSN: 1000-3061 [Print] China |
PMID | 19670635
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adenovirus E2 Proteins
- Vaccines, DNA
- Viral Envelope Proteins
- Viral Vaccines
- glycoprotein E2, classical swine fever virus
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Topics |
- Adenoviridae
(genetics, metabolism)
- Adenovirus E2 Proteins
(genetics, immunology)
- Animals
- Classical Swine Fever
(immunology, prevention & control)
- Classical Swine Fever Virus
(genetics, immunology)
- Genetic Vectors
- Immunization, Secondary
- Replicon
(genetics)
- Semliki forest virus
(genetics, metabolism)
- Swine
- Vaccines, DNA
(immunology)
- Viral Envelope Proteins
(genetics, metabolism)
- Viral Vaccines
(immunology)
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