[Protective effects and mechanism of N-acetylcysteine on cardiac injury induced by ischemia/reperfusion in diabetic rats].

Abstract

AIM:

To study the protective effects and mechanism of N-acetylcysteine on cardiac injury induced by ischemia/reperfusion in diabetic rats.

METHODS:

Diabetes mellitus was induced by intraperitoneal injection of streptozotocin(STZ). The animals were randomly reassigned into sham-operated group, I/R group and I/R+treated with NAC group. The content of GSH and GSSG, and the activity of Caspase-3 were measured. The apoptosis index (AI) by TUNEL staining was calculated. In addition, the apoptosis of Cardiomyocyte was also confirmed by DNA Ladder.

RESULTS:

Treatment with NAC decreased the activity of Caspase-3, the content of GSSG, the values of AI but increased the content of GSH in both non-diabetic and diabetic rats (P<0.05), but there were still significant difference about the values of above parameters between diabetic rats and non-diabetic rats (P<0.05).

CONCLUSION:

NAC can attenuated cardiomyocyte apoptosis by decreasing the activity of Caspase-3 and increasing the content of GSH, which has protective effect on ischemic/reperfused myocardium injury in both non-diabetic and diabetic rats, but the cardioprotective effect is less effective in diabetic rats than that in non-diabetic rats.

• Authors: Shan Zeng, Yi Lin, Jing-fang DI, Zheng Feng
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 25 Issue 8 Pg. 719-21 (Aug 2009) ISSN: 1007-8738 [Print] China
• PMID: 19664398 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetylcysteine

Topics

• Acetylcysteine (administration & dosage)
• Animals
• Diabetes Complications (drug therapy, metabolism)
• Disease Models, Animal
• Female
• Humans
• Ischemia (drug therapy, metabolism)
• Male
• Myocardial Reperfusion Injury (drug therapy, metabolism)
• Myocytes, Cardiac (drug effects, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley