Abstract |
Cloned cDNA encoding the M protein of the porcine transmissible gastroenteritis coronavirus (TGEV) was introduced into a vaccinia virus to examine the function of the amino-terminal signal peptide. The M protein expressed by the recombinant virus was targeted to the Golgi region of infected cells, as is the M protein in cells infected with TGEV. The protein appeared not to undergo processing other than glycosylation. However, the vaccinia-expressed M protein was slightly larger than the protein found in TGEV-infected cells, suggesting that a difference in modification exists between the proteins.
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Authors | B G Hogue, D P Nayak |
Journal | Advances in experimental medicine and biology
(Adv Exp Med Biol)
Vol. 276
Pg. 121-6
( 1990)
ISSN: 0065-2598 [Print] United States |
PMID | 1966402
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Protein Sorting Signals
- Viral Matrix Proteins
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Topics |
- Animals
- Cells, Cultured
- Cloning, Molecular
- Gene Expression
- Genes, Viral
- Male
- Molecular Weight
- Protein Sorting Signals
(genetics)
- Restriction Mapping
- Swine
- Testis
- Transmissible gastroenteritis virus
(genetics)
- Vaccinia virus
(genetics)
- Viral Matrix Proteins
(biosynthesis, genetics, isolation & purification)
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