Release and spread of immunoreactive neurokinin A in the cat spinal cord in a model of acute arthritis.

Abstract

Antibody microprobes were used to study the release of immunoreactive neurokinin A into the spinal cord of anaesthetised cats during and following injection of a knee joint with kaolin and carrageenan. A basal level of immunoreactive neurokinin A was detected prior to any noxious stimuli. Innocuous mechanical joint stimuli (flexion or pressure) did not alter this basal level of release. However, on injection of kaolin and carrageenan into a knee joint, evidence of release into the ipsilateral spinal cord was immediately observed. Initially, immunoreactive neurokinin A was detected in 2 regions: one at the dorsal surface of the spinal cord and the other centred on the superficial dorsal horn. Within 1 h of joint injection, however, immunoreactive neurokinin A was detected throughout the dorsal horn and the adjacent white matter. The extensive spread and persistence of immunoreactive neurokinin A in the spinal cord may underlie some of the prolonged excitability changes evoked by brief noxious stimuli and peripheral inflammation reported by other laboratories.

• Authors: P J Hope, B Jarrott, H G Schaible, R W Clarke, A W Duggan
• Journal: Brain research (Brain Res) Vol. 533 Issue 2 Pg. 292-9 (Nov 19 1990) ISSN: 0006-8993 [Print] Netherlands
• PMID: 1963110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Molecular Probes
• Kaolin
• Neurokinin A
• Carrageenan

Topics

• Acute Disease
• Animals
• Arthritis (chemically induced, metabolism)
• Autoradiography
• Carrageenan
• Cats
• Disease Models, Animal
• Kaolin
• Molecular Probes
• Neurokinin A (metabolism)
• Spinal Cord (metabolism)
• Stress, Mechanical