Abstract | BACKGROUND AND OBJECTIVE: METHODS: The content and activity of DNA- PKcs in five NSCLC cell lines A549, H1299, L78, PGCL3 and H460 were measured by Western blot and the DNA-PK activity assay. Cell survival was analyzed using clonogenic formation assay. RESULTS: The radiosensitivities of five NSCLC cell lines were different. The values of survival fraction at 2 Gy (SF2) were 0.74 in A549 cells, 0.25 in H1299 cells, 0.21 in H460 cells, 0.48 in PGCL3 cells, and 0.58 in L78 cells. The protein levels of DNA- PKcs were 3.26+/-0.98 in A549 cells, 0.51+/-0.07 in L78 cells, 0.51+/-0.11 in H1299 cells, 0.86+/-0.23 in H460 cells, and 2.60+/-0.76 in PGCL3 cells. The activity values of DNA- PKcs were 8.30+/-1.03 in A549 cells, 2.45+/-0.52 in H1299 cells, 0.11+/-0.02 in H460 cells, 4.13+/-0.87 in PGCL3 cells, and 0.42+/-0.07 in L78 cells. In adenocarcinoma and large cell carcinoma cell lines, SF2 were correlated to DNA- PKcs content (P<0.05, r=0.95) and activity (P=0.03, r=0.98). CONCLUSION:
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Authors | Yi Pan, Wei-Xiong Li, Jun-Ming Li, Jian-Quan Zhu, Yi-Qiang Liang, Ai-Lin Guo |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 28
Issue 7
Pg. 714-7
(Jul 2009)
China |
PMID | 19624897
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Activated Protein Kinase
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Topics |
- Adenocarcinoma
(metabolism, pathology)
- Carcinoma, Large Cell
(metabolism, pathology)
- Carcinoma, Non-Small-Cell Lung
(metabolism, pathology)
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Catalytic Domain
- Cell Line, Tumor
- Cell Survival
(radiation effects)
- DNA-Activated Protein Kinase
(metabolism)
- Enzyme Activation
(radiation effects)
- Humans
- Lung Neoplasms
(metabolism, pathology)
- Particle Accelerators
- Radiation Tolerance
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