Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.

Abstract

Young mink kits (n=8) were vaccinated with DNA plasmids encoding the viral haemagglutinin protein (H) of a vaccine strain of Canine distemper virus (CDV). Virus neutralising (VN) antibodies were induced after 2 immunisations and after the third immunisation all kits had high VN antibody titres. The VN antibody titres remained high for more than 4 months and the mink were protected against viraemia, lymphopenia, clinical disease and changes in the percentage of IFN-gamma producing peripheral blood leucocytes after challenge inoculation with a recent wild type strain of CDV. Essentially, these results demonstrate that early life DNA vaccination with the H gene of a CDV vaccine strain induced robust protective immunity against a recent wild type CDV.

• Authors: Trine Hammer Jensen, Line Nielsen, Bent Aasted, Merete Blixenkrone-Møller
• Journal: Vaccine (Vaccine) Vol. 27 Issue 38 Pg. 5178-83 (Aug 20 2009) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 19596418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Hemagglutinins, Viral
• Vaccines, DNA
• Viral Vaccines
• Interferon-gamma

Topics

• Animals
• Antibodies, Viral (blood, immunology)
• Chlorocebus aethiops
• Distemper (immunology, prevention & control)
• Distemper Virus, Canine (immunology)
• Hemagglutinins, Viral (immunology)
• Interferon-gamma (immunology)
• Mink (immunology, virology)
• Neutralization Tests
• Plasmids
• Vaccines, DNA (immunology)
• Vero Cells
• Viral Vaccines (immunology)