Cardenolide glycosides of Thevetia peruviana and triterpenoid saponins of Sapindus emarginatus as TRAIL resistance-overcoming compounds.

Abstract

A screening study for TRAIL resistance-overcoming activity was carried out, and activity-guided fractionations of Thevetia peruviana and Sapindus emarginatus led to the isolation of four cardenolide glycosides (1-4) and four triterpenoid saponins (5-8), respectively. In particular, cardenolide glycosides (1 and 2) from T. peruviana were shown to have a significant reversal effect on TRAIL resistance in human gastric adenocarcinoma cells, and real-time PCR showed that thevefolin (2) enhanced mRNA expression of death receptor 4 (DR4) and DR5. In addition, 1H and 13C NMR characterizations are shown for thevefolin (2) for the first time.

• Authors: Takashi Miyagawa, Takashi Ohtsuki, Takashi Koyano, Thaworn Kowithayakorn, Masami Ishibashi
• Journal: Journal of natural products (J Nat Prod) Vol. 72 Issue 8 Pg. 1507-11 (Aug 2009) ISSN: 1520-6025 [Electronic] United States
• PMID: 19594141 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardenolides
• Receptors, TNF-Related Apoptosis-Inducing Ligand
• Saponins
• TNF-Related Apoptosis-Inducing Ligand

Topics

• Base Sequence
• Cardenolides (chemistry, isolation & purification, pharmacology)
• Humans
• Molecular Structure
• Nuclear Magnetic Resonance, Biomolecular
• Plants, Medicinal (chemistry)
• Receptors, TNF-Related Apoptosis-Inducing Ligand (genetics)
• Sapindus (chemistry)
• Saponins (chemistry, isolation & purification, pharmacology)
• Stomach Neoplasms (metabolism)
• TNF-Related Apoptosis-Inducing Ligand (drug effects)
• Thailand
• Thevetia (chemistry)
• Tumor Cells, Cultured