Cytokine and molecular analyses of intraocular lymphoma.

Abstract

PURPOSE:

The authors investigate the efficacy of using the cytokine levels and clonal heavy-chain immunoglobulin (IgH) gene rearrangements in the vitreous as adjunctive tools to diagnose intraocular lymphoma (IOL).

METHODS:

The IL-10 and IL-6 levels and IgH gene rearrangements were analyzed in vitreous samples from 8 cases of IOL and in 14 uveitis patients.

RESULTS:

The level of IL-10 with an IL-10/IL-6 ratio > 1 was significantly higher in all eyes with IOL. B-cell monoclonality was detected in only 5 of 8 eyes with IOL.

CONCLUSIONS:

The measurements of the levels of cytokines are valuable as a reliable biomarker.

• Authors: Kouichi Ohta, Kenji Sano, Hiroki Imai, Takanobu Kikuchi
• Journal: Ocular immunology and inflammation (Ocul Immunol Inflamm) 2009 May-Jun Vol. 17 Issue 3 Pg. 142-7 ISSN: 1744-5078 [Electronic] England
• PMID: 19585355 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• IL10 protein, human
• IL6 protein, human
• Immunoglobulin Heavy Chains
• Interleukin-6
• Interleukin-10

Topics

• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Rearrangement, B-Lymphocyte, Heavy Chain (genetics)
• Humans
• Immunoglobulin Heavy Chains (genetics)
• Interleukin-10 (metabolism)
• Interleukin-6 (metabolism)
• Luminescent Measurements
• Lymphoma, B-Cell (diagnosis, genetics, metabolism)
• Male
• Middle Aged
• Polymerase Chain Reaction
• Retinal Neoplasms (diagnosis, genetics, metabolism)
• Uveitis (diagnosis, genetics, metabolism)
• Vitrectomy
• Vitreous Body (metabolism, pathology)