Abstract | BACKGROUND: Hemidesmosomal proteins may become targets of autoimmunity in subepidermal blistering diseases. Well-known recognized autoantigens are the intracellular plaque protein BP230, the transmembrane BP180 and its shed ectodomain LAD-1. OBJECTIVES: To establish the prevalence of autoimmunity against plectin, another intracellular plaque protein, and to investigate its antigenic sites. METHODS: Two hundred and eighty-two patients with subepidermal blistering diseases, investigated by routine immunoblot analysis for possible antiplectin antibodies, were included in the study. Epitope mapping was performed using recombinantly produced overlapping plectin domains from the actin-binding domain to the rod domain. The COOH-terminal region of plectin was not included in the study. RESULTS: CONCLUSIONS:
|
Authors | J J A Buijsrogge, M C J M de Jong, G J Kloosterhuis, M H Vermeer, J Koster, A Sonnenberg, M F Jonkman, H H Pas |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 161
Issue 4
Pg. 762-71
(Oct 2009)
ISSN: 1365-2133 [Electronic] England |
PMID | 19566666
(Publication Type: Journal Article)
|
Chemical References |
- Autoantibodies
- Autoantigens
- Immunodominant Epitopes
- Immunoglobulin A
- Immunoglobulin G
- Plectin
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Autoantibodies
(immunology)
- Autoantigens
(immunology)
- Epitope Mapping
- Female
- Humans
- Immunoblotting
- Immunodominant Epitopes
(immunology)
- Immunoglobulin A
(immunology)
- Immunoglobulin G
(immunology)
- Infant
- Male
- Middle Aged
- Pemphigoid, Bullous
(immunology)
- Plectin
(immunology)
|