[Activation of nuclear factor-kappaB and aberrant gene expression of interleukin 6 in middle ear cholesteatoma].

Abstract

OBJECTIVE:

To establish the activation of NF-kappaB in middle ear cholesteatoma, discuss the relationship of NF-kappaB and the gene expression of IL-6 and explore the pathogenesis of middle ear cholesteatoma.

METHODS:

Ten cases of cholesteatoma and 6 cases of normal external meatal skin were obtained from middle ear surgery. The NF-kappaB DNA binding activity and the mRNA level of IL-6 in these two kinds of tissues were detected by electrophoretic motility shift assay (EMSA) and Reverse transcription polymerase chain reaction (RT-PCR) respectively. The relationship of NF-kappaB DNA binding activity and the mRNA level of IL-6 were analyzed.

RESULTS:

The NF-kappaB DNA binding activities of cholesteatoma [(15.9 +/- 8.2)%] were higher than those in normal skin [(1.36 +/- 0.94)%, t = 3.502, P < 0.05]. The expression of IL-6 mRNA was increased significantly in patients with cholesteatoma, as compared with that in the control specimens (t = 2.166, P < 0.05) and had a significant positive correlation with NF-kappaB DNA binding activity (r = 0.752, P < 0.05).

CONCLUSIONS:

The IL-6 mRNA expression in cholesteatoma is closely related with the activity of NF-kappaB. It is tempting to speculate that NF-kappaB play a key role in the activation of cytokine in cholesteatoma.

• Authors: Yu Xu, Ze-zhang Tao, Qing-quan Hua, Xin-chun Wang, Bo-kui Xiao, Shi-ming Chen
• Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 44 Issue 3 Pg. 192-6 (Mar 2009) ISSN: 1673-0860 [Print] China
• PMID: 19558856 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IL6 protein, human
• Interleukin-6
• NF-kappa B
• RNA, Messenger

Topics

• Cholesteatoma, Middle Ear (genetics, metabolism)
• Gene Expression Regulation
• Humans
• Interleukin-6 (genetics, metabolism)
• NF-kappa B (metabolism)
• RNA, Messenger (genetics)