Abstract |
An undiagnosed pheochromocytoma may result in life-threatening consequences. The diagnosis of pheochromocytoma is based on the overproduction of catecholamines. Highly sensitive biochemical assays are essential to avoid false-negative results. Determinations of 24-h urinary epinephrine and norepinephrine levels are established diagnostic tools. However, they may be falsely negative in patients with a biochemically-silent or periodically-secreting pheochromocytoma. Metanephrines, which are metabolites of catecholamines, have been suggested as an alternative diagnostic tool. Urinary metanephrines are determined by high-pressure liquid chromatography (HPLC) in an increasing number of laboratories, whereas plasma metanephrines measured by HPLC are available in specialised centres only. The different HPLC methods may be cost- and time-intensive. Immunoassays such as radio- or enzyme-immunoassays may be alternative procedures. Measurement of metanephrines instead of catecholamines by either technique improved the diagnostic accuracy for the diagnosis of pheochromocytomas. Determination of plasma free metanephrines demonstrated a higher accuracy than their urinary counterparts. The use of immunoassays may be an alternative to the laborious HPLC, although the method needs to be evaluated in more detail.
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Authors | N Unger, T Deutschbein, M K Walz, K Mann, S Petersenn |
Journal | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
(Horm Metab Res)
Vol. 41
Issue 9
Pg. 676-9
(Sep 2009)
ISSN: 1439-4286 [Electronic] Germany |
PMID | 19557670
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
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Topics |
- Adrenal Gland Neoplasms
(blood, diagnosis, urine)
- Humans
- Immunoassay
(economics, methods)
- Metanephrine
(blood, urine)
- Pheochromocytoma
(blood, diagnosis, urine)
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