HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Hyperuricemia, oxidative stress, and carotid artery tone in experimental renal insufficiency.

AbstractBACKGROUND:
Hyperuricemia may play a role in the pathogenesis of cardiovascular disease, but uric acid is also a significant antioxidant. We investigated the effects of oxonic acid-induced hyperuricemia on carotid artery tone in experimental renal insufficiency.
METHODS:
Three weeks after 5/6 nephrectomy (NX) or Sham operation, male Sprague-Dawley rats were allocated to 2.0% oxonic acid or control diet for 9 weeks. Blood pressure was monitored using tail cuff, isolated arterial rings were examined using myographs, and blood and urine samples were taken, as appropriate. Oxidative stress and antioxidant status were evaluated by measuring urinary 8-isoprostaglandin F(2 alpha) (8-iso-PGF(2 alpha)) excretion and plasma total peroxyl radical-trapping capacity (TRAP), respectively.
RESULTS:
Plasma creatinine was elevated twofold in NX rats, but neither NX nor oxonic acid diet influenced blood pressure. Urinary 8-iso-PGF(2 alpha) excretion was increased over 2.5-fold in NX rats on control diet. Oxonic acid diet increased plasma uric acid 2-3-fold, TRAP 1.5-fold, and reduced urinary 8-iso-PGF(2 alpha) excretion by 60-90%. Carotid vasorelaxation to acetylcholine in vitro, which could be abolished by nitric oxide (NO) synthase inhibition, was reduced following NX, whereas maximal response to acetylcholine was augmented in hyperuricemic NX rats. Vasorelaxation to nitroprusside was impaired in NX rats, whereas oxonic acid diet increased sensitivity also to nitroprusside in NX rats.
CONCLUSIONS:
Oxonic acid-induced hyperuricemia reduced oxidative stress in vivo, as evaluated using urinary 8-iso-PGF(2 alpha) excretion, increased plasma TRAP, and improved NO-mediated vasorelaxation in the carotid artery in experimental renal insufficiency.
AuthorsVenla Kurra, Arttu Eräranta, Pasi Jolma, Tuija I Vehmas, Asko Riutta, Eeva Moilanen, Anna Tahvanainen, Jarkko Kalliovalkama, Onni Niemelä, Juhani Myllymäki, Jukka Mustonen, Ilkka Pörsti
JournalAmerican journal of hypertension (Am J Hypertens) Vol. 22 Issue 9 Pg. 964-70 (Sep 2009) ISSN: 1941-7225 [Electronic] United States
PMID19521342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Peroxides
  • Uric Acid
  • 8-epi-prostaglandin F2alpha
  • perhydroxyl radical
  • Oxonic Acid
  • Creatinine
  • Dinoprost
  • NG-Nitroarginine Methyl Ester
Topics
  • Animals
  • Carotid Arteries (drug effects, physiology)
  • Creatinine (blood)
  • Dinoprost (analogs & derivatives, urine)
  • Hyperuricemia (chemically induced, physiopathology)
  • Male
  • NG-Nitroarginine Methyl Ester (pharmacology)
  • Nephrectomy
  • Oxidative Stress (drug effects)
  • Oxonic Acid
  • Peroxides (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Renal Insufficiency (physiopathology)
  • Uric Acid (blood)
  • Vasodilation (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: