Abstract | BACKGROUND: METHODS: Three weeks after 5/6 nephrectomy (NX) or Sham operation, male Sprague-Dawley rats were allocated to 2.0% oxonic acid or control diet for 9 weeks. Blood pressure was monitored using tail cuff, isolated arterial rings were examined using myographs, and blood and urine samples were taken, as appropriate. Oxidative stress and antioxidant status were evaluated by measuring urinary 8-isoprostaglandin F(2 alpha) (8-iso-PGF(2 alpha)) excretion and plasma total peroxyl radical-trapping capacity (TRAP), respectively. RESULTS: Plasma creatinine was elevated twofold in NX rats, but neither NX nor oxonic acid diet influenced blood pressure. Urinary 8-iso-PGF(2 alpha) excretion was increased over 2.5-fold in NX rats on control diet. Oxonic acid diet increased plasma uric acid 2-3-fold, TRAP 1.5-fold, and reduced urinary 8-iso-PGF(2 alpha) excretion by 60-90%. Carotid vasorelaxation to acetylcholine in vitro, which could be abolished by nitric oxide ( NO) synthase inhibition, was reduced following NX, whereas maximal response to acetylcholine was augmented in hyperuricemic NX rats. Vasorelaxation to nitroprusside was impaired in NX rats, whereas oxonic acid diet increased sensitivity also to nitroprusside in NX rats. CONCLUSIONS:
Oxonic acid-induced hyperuricemia reduced oxidative stress in vivo, as evaluated using urinary 8-iso-PGF(2 alpha) excretion, increased plasma TRAP, and improved NO-mediated vasorelaxation in the carotid artery in experimental renal insufficiency.
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Authors | Venla Kurra, Arttu Eräranta, Pasi Jolma, Tuija I Vehmas, Asko Riutta, Eeva Moilanen, Anna Tahvanainen, Jarkko Kalliovalkama, Onni Niemelä, Juhani Myllymäki, Jukka Mustonen, Ilkka Pörsti |
Journal | American journal of hypertension
(Am J Hypertens)
Vol. 22
Issue 9
Pg. 964-70
(Sep 2009)
ISSN: 1941-7225 [Electronic] United States |
PMID | 19521342
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Peroxides
- Uric Acid
- 8-epi-prostaglandin F2alpha
- perhydroxyl radical
- Oxonic Acid
- Creatinine
- Dinoprost
- NG-Nitroarginine Methyl Ester
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Topics |
- Animals
- Carotid Arteries
(drug effects, physiology)
- Creatinine
(blood)
- Dinoprost
(analogs & derivatives, urine)
- Hyperuricemia
(chemically induced, physiopathology)
- Male
- NG-Nitroarginine Methyl Ester
(pharmacology)
- Nephrectomy
- Oxidative Stress
(drug effects)
- Oxonic Acid
- Peroxides
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Renal Insufficiency
(physiopathology)
- Uric Acid
(blood)
- Vasodilation
(drug effects)
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