Abstract | BACKGROUND/AIMS: METHODS: Progressive mesangioproliferative nephritis was induced in male Wistar rats by uninephrectomy and anti-Thy1.1 antibody injection. Rats were treated with either vehicle ( phosphate-buffered saline; n = 10) or nonspecific human polyclonal IgG ( IgG; n = 10) on days 3, 10 and 17 after disease induction and sacrificed on day 56. RESULTS: During the experiment, IgG treatment prevented weight loss and had a beneficial effect on the rise in serum creatinine and the decline of creatinine clearance. At sacrifice, a significantly lower number of IgG-treated rats had tripled their creatinine or halved their creatinine clearance. Moreover, during the 56 days of follow-up, the IgG-treated group exhibited reduced mortality due to renal failure. At sacrifice, IgG-treated rats had reduced indices of renal fibrosis. CONCLUSIONS:
IgG treatment is an effective treatment approach in rats with progressive glomerulonephritis. Our data also indicate that studies using specific antibodies need to be controlled for nonspecific IgG effects.
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Authors | Peter Boor, Uta Kunter |
Journal | Kidney & blood pressure research
(Kidney Blood Press Res)
Vol. 32
Issue 3
Pg. 169-74
( 2009)
ISSN: 1423-0143 [Electronic] Switzerland |
PMID | 19506398
(Publication Type: Journal Article)
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Copyright | Copyright (c) 2009 S. Karger AG, Basel. |
Chemical References |
- Immunoglobulins, Intravenous
- Creatinine
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Topics |
- Animals
- Creatinine
(blood, metabolism)
- Glomerulonephritis, Membranoproliferative
(drug therapy)
- Immunoglobulins, Intravenous
(pharmacology, therapeutic use)
- Male
- Rats
- Rats, Wistar
- Survival Rate
- Treatment Outcome
- Weight Loss
(drug effects)
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