[Mutation patterns in the RT region of hepatitis B virus P gene in patients treated with nucleoside/nucleotide analogs].

Abstract

OBJECTIVE:

To analyze mutation patterns in the RT region of hepatitis B virus (HBV) P gene in patients treated with nucleoside or nucleotide analogs.

METHODS:

Viral DNA was extracted from 227 serum samples of chronic hepatitis B patients from September, 2005 to March, 2007. The RT region of HBV P gene was PCR-amplified and sequenced.

RESULTS:

Known resistant mutations were found in 111 cases (48.9%) among 227 samples, 75 cases with clear therapy history. Novel mutations, including A222T, L229V and S256C, were also found. The incidence of multi-drug resistance in patients sequentially treated with lamivudine and adefovir was 25% (4/16), and none of the patients treated with lamivudine plus adefovir in combination shown multi-drug resistance.

CONCLUSION:

The patterns of mutation is complex in nucleotide analogue treated patients. Switching to adefovir in lamivudine resistant patients may lead to multi-drug resistance. Novel mutations identified in this study need further investigation.

• Authors: Jun DENG, Dong-hua ZHANG, De-min YU, Li CHEN, Xin-xin ZHANG
• Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi) Vol. 17 Issue 5 Pg. 342-5 (May 2009) ISSN: 1007-3418 [Print] China
• PMID: 19497198 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• DNA, Viral
• Gene Products, pol
• P protein, Hepatitis B virus
• Reverse Transcriptase Inhibitors

Topics

• Adolescent
• Adult
• Aged
• Antiviral Agents (pharmacology, therapeutic use)
• DNA Mutational Analysis
• DNA, Viral (genetics)
• Drug Resistance, Viral
• Female
• Gene Products, pol (genetics)
• Genes, Viral
• Genetic Variation
• Hepatitis B virus (drug effects, genetics)
• Hepatitis B, Chronic (drug therapy, genetics, virology)
• Humans
• Male
• Middle Aged
• Reverse Transcriptase Inhibitors (pharmacology, therapeutic use)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Young Adult