Proteomic analysis of the rat ovary following chronic low-dose exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitously distributed endocrine-disrupting chemical and reproductive toxicant. In order to elucidate low-dose TCDD-mediated effects on reproductive or endocrine functions, female Sprague-Dawley rats were orally administered various concentrations (20, 50, or 125 ng/kg once weekly) TCDD for 29 wk. A proteomic analysis of the ovaries by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry showed distinct changes in the levels of several proteins that are relevant markers of TCDD toxicity. Serum estradiol (E2) levels of TCDD-treated animals were markedly lower than control. There were no significant differences in bone mineral density (BMD) of femurs. The body weight of the 125-ng/kg TCDD group was significantly decreased relative to control and there was also a significant reduction in absolute and relative ovarian weights. Expressions of selenium binding protein 2, glutathione S-transferase mu type 3, Lrpap1 protein, NADPH, and peptidylprolyl isomerase D were upregulated, while prohibitin and N-ethylmaleimide-sensitive factor expression levels were downregulated. Data provide further insight into the mechanisms by which TCDD disrupts ovarian function by indicating which differential protein expressions following low-dose TCDD exposure.

• Authors: Xi Chen, Xiao-ming Ma, Shi-wei Ma, Pieter-Jan Coenraads, Chun-mei Zhang, Jing Liu, Li-jun Zhao, Min Sun, Nai-jun Tang
• Journal: Journal of toxicology and environmental health. Part A (J Toxicol Environ Health A) Vol. 72 Issue 11-12 Pg. 717-26 ( 2009) ISSN: 1528-7394 [Print] England
• PMID: 19492234 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Environmental Pollutants
• Isoenzymes
• LDL-Receptor Related Protein-Associated Protein
• Polychlorinated Dibenzodioxins
• Prohibitins
• Repressor Proteins
• Selenbp1 protein, rat
• Selenium-Binding Proteins
• Estradiol
• NADP
• Glutathione Transferase
• glutathione transferase M3-3
• N-Ethylmaleimide-Sensitive Proteins
• Peptidylprolyl Isomerase

Topics

• Administration, Oral
• Animals
• Down-Regulation
• Environmental Pollutants (administration & dosage, toxicity)
• Estradiol (metabolism)
• Female
• Glutathione Transferase (metabolism)
• Isoenzymes (metabolism)
• LDL-Receptor Related Protein-Associated Protein
• N-Ethylmaleimide-Sensitive Proteins (metabolism)
• NADP (metabolism)
• Organ Size (drug effects)
• Ovary (drug effects, metabolism)
• Peptidylprolyl Isomerase (metabolism)
• Polychlorinated Dibenzodioxins (administration & dosage, toxicity)
• Prohibitins
• Proteomics
• Rats
• Rats, Sprague-Dawley
• Repressor Proteins (metabolism)
• Selenium-Binding Proteins
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Up-Regulation