All in the timing: a comparison between the cardioprotection induced by H2S preconditioning and post-infarction treatment.

Abstract

In the current study, we investigated the delayed cardioprotection induced by H(2)S preconditioning in an in vivo rat model of myocardial infarction. Assessment of infarct size revealed that a single bolus of NaHS (a donor of H(2)S, at 0.1-10 micromol/kg body weight) administered 1 day before myocardial infarction produced a strong infarct-limiting effect. A time course study demonstrated that the protection lasted at least 3 days after the preconditioning stimulus. We further compared the effect of H(2)S preconditioning with post-infarction treatment. Although injection of NaHS (1 micromol/kg once daily) for 3 days after myocardial infarction also significantly decreased infarct size, the protective effect was significantly lower than that afforded by H(2)S preconditioning. A combination of both preconditioning and post-treatment did not produce a stronger protection compared with H(2)S preconditioning alone. Pretreatment with chelerythrine chloride (5 mg/kg, i.p.), a protein kinase C (PKC) inhibitor, 15 min before NaHS administration blocked the infarct-sparing effect of H(2)S preconditioning. In conclusion, the current study provided the first evidence that H(2)S preconditioning produces strong late cardioprotection through a PKC-dependent mechanism. Such protection could not be reproduced by H(2)S treatment after the infarction occurred. A combination of both preconditioning and post-treatment does not provide additional benefit and hence is not necessary when the access to preconditioning has been secured.

• Authors: Ting-Ting Pan, Yong Qian Chen, Jin-Song Bian
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 616 Issue 1-3 Pg. 160-5 (Aug 15 2009) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 19482017 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Kinase Inhibitors
• Protein Kinase C
• Hydrogen Sulfide

Topics

• Animals
• Dose-Response Relationship, Drug
• Heart (drug effects)
• Hydrogen Sulfide (administration & dosage, pharmacology)
• Ischemic Preconditioning, Myocardial
• Male
• Myocardial Infarction (drug therapy, pathology, prevention & control)
• Myocardium (pathology)
• Protein Kinase C (antagonists & inhibitors)
• Protein Kinase Inhibitors (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Time Factors