Expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands: relationship with clinical and histologic findings.

Abstract

OBJECTIVE:

The aim was to examine the relationship between clinicopathological features and immunoexpression of hMLH1 and hMSH2 proteins in pleomorphic adenoma (PA) of minor salivary glands.

STUDY DESIGN:

Paraffin-embedded samples of typically benign PA lesions (n = 35) were prepared for histologic and immunohistochemical assessment. Based on the clinicopathologic features, the samples were categorized into low- and high-risk subtypes for their estimated potential for malignant transformation.

RESULTS:

Immunohistochemical analysis revealed strong correlations regarding the expression estimation and staining-intensity distribution (SID) scores between the two proteins. Although there was no relationship between marker immunoexpression and SID scores regarding clinical parameters, statistically significant variations for these parameters were evident regarding some histologic criteria or for risk stratification subtypes.

CONCLUSION:

The findings of this study could suggest the relationship of a DNA mismatch repair deficiency with high-risk subtypes of PA and the implication of its role in the origin and progression of these subtypes.

• Authors: Sergio Iván Tobón-Arroyave, Gloria Amparo Flórez-Moreno, Juan Fernando Jaramillo-Cárdenas, Juan David Arango-Uribe, Diana María Isaza-Guzmán, Javier Rendón-Henao, POPCAD Research Group
• Journal: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 108 Issue 2 Pg. 227-36 (Aug 2009) ISSN: 1528-395X [Electronic] United States
• PMID: 19464205 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• Biomarkers, Tumor
• MLH1 protein, human
• Nuclear Proteins
• MSH2 protein, human
• MutL Protein Homolog 1
• MutS Homolog 2 Protein

Topics

• Adaptor Proteins, Signal Transducing (metabolism)
• Adenoma, Pleomorphic (metabolism)
• Adolescent
• Adult
• Aged
• Biomarkers, Tumor (metabolism)
• Child
• DNA Mismatch Repair (physiology)
• Female
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• MutL Protein Homolog 1
• MutS Homolog 2 Protein (metabolism)
• Nuclear Proteins (metabolism)
• Salivary Gland Neoplasms (metabolism, pathology)
• Salivary Glands, Minor (metabolism, pathology)