Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector.

Abstract

Erysipelothrix rhusiopathiae Koganei 65-0.15 strain, the live swine erysipelas vaccine for subcutaneous injection, has been shown to colonize the tonsils of pigs after oral inoculation. We thus evaluated the possible use of the strain as a vector for oral vaccination against mycoplasmal pneumonia of swine. Recombinant E. rhusiopathiae strains expressing the C-terminal domain of the P97 adhesin of Mycoplasma hyopneumoniae were constructed and examined for vaccine efficacy in mice and pigs. Mice subcutaneously inoculated with the recombinant strains were protected from challenge exposure to a virulent E. rhusiopathiae. Administration of milk replacer containing recombinant E. rhusiopathiae expressing the M. hyopneumoniae protein protected pigs from death after exposure to E. rhusiopathiae and significantly reduced the severity of pneumonic lung lesions caused by infection with M. hyopneumoniae.

• Authors: Yohsuke Ogawa, Eiji Oishi, Yoshihiro Muneta, Akiyuki Sano, Hirokazu Hikono, Tomoyuki Shibahara, Yukio Yagi, Yoshihiro Shimoji
• Journal: Vaccine (Vaccine) Vol. 27 Issue 33 Pg. 4543-50 (Jul 16 2009) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 19433128 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adhesins, Bacterial
• Antibodies, Bacterial
• Bacterial Vaccines
• P97 protein, Mycoplasma
• Vaccines, Attenuated

Topics

• Adhesins, Bacterial (immunology)
• Administration, Oral
• Animals
• Antibodies, Bacterial (blood, immunology)
• Bacterial Vaccines (immunology)
• Erysipelothrix (immunology, isolation & purification)
• Female
• Infusions, Subcutaneous
• Lung (microbiology, pathology)
• Mice
• Mycoplasma hyopneumoniae (immunology)
• Palatine Tonsil (immunology, microbiology)
• Pneumonia of Swine, Mycoplasmal (immunology, prevention & control)
• Swine
• Vaccines, Attenuated (immunology)