Abstract | BACKGROUND: RESULTS: Pharmacological inhibition of either p38 or extracellular signal-regulated kinase (ERK) in the injured DRG, which led to suppression of the development of tactile allodynia, did not affect cPLA2 phosphorylation and translocation after nerve injury. By contrast, a CaMKII inhibitor prevented the development and expression of nerve injury-induced tactile allodynia and reduced both the level of cPLA2 phosphorylation and the number of DRG neurons showing translocated cPLA2 in response to nerve injury. Applying ATP to cultured DRG neurons increased the level of both phosphorylated cPLA2 and CaMKII in the vicinity of the plasma membrane and caused physical association of these two proteins. In addition, ATP-stimulated cPLA2 and CaMKII phosphorylation were inhibited by both a selective P2X3R/P2X2+3R antagonist and a nonselective voltage-dependent Ca2+ channel ( VDCC) blocker. CONCLUSION: These results suggest that CaMKII, but not MAPKs, has an important role in cPLA2 activation following peripheral nerve injury, probably through P2X3R/P2X2+3R and VDCCs in primary afferent neurons.
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Authors | Shigeo Hasegawa, Yuta Kohro, Makoto Tsuda, Kazuhide Inoue |
Journal | Molecular pain
(Mol Pain)
Vol. 5
Pg. 22
(May 02 2009)
ISSN: 1744-8069 [Electronic] United States |
PMID | 19409102
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Receptors, Purinergic P2
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
- p38 Mitogen-Activated Protein Kinases
- Phospholipases A2, Cytosolic
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Topics |
- Animals
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
(antagonists & inhibitors, metabolism)
- Ganglia, Spinal
(cytology, enzymology, metabolism)
- Male
- Models, Animal
- Neurons, Afferent
(enzymology, metabolism)
- Pain Measurement
- Phospholipases A2, Cytosolic
(metabolism)
- Rats
- Receptors, Purinergic P2
(metabolism)
- Spinal Cord Injuries
(enzymology, metabolism)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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