Abstract | OBJECTIVE: METHODS: One hundred and twenty-one 18-day-old Wistar rats were randomly injected with LPS (4 mg/kg; n=55), LPS (4 mg/kg)+ glutamine (1 mL/kg) (n=55), or normal saline (control group; n=11). The two LPS groups were subdivided into five groups sacrificed at 2, 4, 6, 24 and 72 hrs after administration (n=11 each). ERK-2 and p38MAPK mRNA and protein expression in the kidney were measured by RT-PCR and immunohistochemistry. RESULTS: Compared to the control group, the mRNA and protein expression of both ERK-2 and p38MAPK in the LPS group significantly increased 2, 4, 6, 24 and 72 hrs after administration (P<0.01), and reached a peak at 6 hrs (P<0.01). In the LPS+glutamine group, the trend of ERK-2 and p38MAPK expression was similar to the LPS group but their expression levels were significantly lower than those in the LPS group at all time points (P<0.05 or 0.01). CONCLUSIONS: Both ERK-2 and p38MAPK expression increased in young rats with LPS-induced endotoxemia. Glutamine alleviates renal injuries possibly by decreasing the expression of both.
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Authors | Ping Zhou, Yu-Bin Wu, Cheng-Guang Zhao, Xu Teng, Li Li, Bing Li |
Journal | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
(Zhongguo Dang Dai Er Ke Za Zhi)
Vol. 11
Issue 4
Pg. 301-5
(Apr 2009)
ISSN: 1008-8830 [Print] China |
PMID | 19374817
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- RNA, Messenger
- Glutamine
- Extracellular Signal-Regulated MAP Kinases
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Endotoxemia
(enzymology, pathology)
- Extracellular Signal-Regulated MAP Kinases
(analysis, genetics)
- Female
- Glutamine
(pharmacology)
- Immunohistochemistry
- Kidney
(enzymology)
- Lipopolysaccharides
(toxicity)
- Male
- RNA, Messenger
(analysis)
- Rats
- Rats, Wistar
- p38 Mitogen-Activated Protein Kinases
(analysis, genetics)
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