Modulation of low-density lipoprotein-induced inhibition of intercellular communication by antioxidants and high-density lipoproteins.

Abstract

In order to study the capacity of antioxidants and high-density lipoproteins (HDL) to modulate the effects of low-density lipoprotein (LDL) on intercellular communication, arterial smooth muscle cells and a dye transfer method were used. LDL, in contrast to HDL, inhibited the communication between arterial smooth muscle cells from human umbilical cord and thoracic aorta in a dose-dependent manner. LDL, which can be oxidized, as detected by the lipid-peroxidation assay and gel electrophoresis, did not influence cell-cell communication in the presence of the antioxidants butylated hydroxytoluene (BHT), alpha-tocopherol and glutathione. The results suggest that LDL must undergo oxidative modification before it can influence intercellular communication. Like antioxidants, HDL diminished the LDL-induced inhibition of cell-cell communication. This study suggests that the modulation of gap-junctional communication by the balance of HDL and LDL in plasma may influence atherogenesis.

• Authors: R M Zwijsen, L H de Haan, J A Kuivenhoven, I C Nusselder
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 29 Issue 9 Pg. 615-20 (Sep 1991) ISSN: 0278-6915 [Print] England
• PMID: 1937292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Lipoproteins, HDL
• Lipoproteins, LDL
• Vitamin E
• Butylated Hydroxytoluene

Topics

• Antioxidants (pharmacology)
• Arteriosclerosis (etiology)
• Butylated Hydroxytoluene (pharmacology)
• Cell Communication (drug effects)
• Cells, Cultured
• Humans
• Lipoproteins, HDL (pharmacology)
• Lipoproteins, LDL (pharmacology)
• Male
• Middle Aged
• Vitamin E (pharmacology)