Abstract |
This study was undertaken to investigate whether serum obtained from ocular MG with undetectable antibodies contains antibodies which bind to normal motor end-plates immunohistochemically. Nineteen patients with ocular MG were studied. Anti-AChR antibodies in serum were assayed by an immunoprecipitation method using human junctional AChR as the antigen. Anti-AChR antibodies in serum which bind to the junctional AChR at the motor end-plates were detected immunohistochemically by incubating the muscle with each serum. Bound IgG was detected by peroxidase labeled protein A (P-PA). IgG deposit at the own limb muscle motor end-plates (biceps brachii) was also detected by P-PA. Anti-AChR antibodies in serum were positive in 9 out of 19 patients and IgG antibodies bound to the junctional AChR were demonstrated in 16 of 19 patients. IgG bound to own end-plates was observed in 13 of 14 patients studied. In 2 patients IgG was detected at the own end-plates, but not at the not-self end-plates. These findings indicate that detection of IgG at the limb muscle end-plates serves for the diagnosis of ocular MG with undetectable antibodies in serum and anti-AChR antibodies in some patients may react exclusively with the autologous AChR.
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Authors | I Kinoshita, M Tsujihata, M Motomura, T Yoshimura, S Nagataki |
Journal | Rinsho shinkeigaku = Clinical neurology
(Rinsho Shinkeigaku)
Vol. 31
Issue 5
Pg. 500-4
(May 1991)
ISSN: 0009-918X [Print] Japan |
PMID | 1934762
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Autoantibodies
- Immunoglobulin G
- Receptors, Cholinergic
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Topics |
- Adolescent
- Adult
- Aged
- Autoantibodies
(analysis)
- Child
- Female
- Humans
- Immunoglobulin G
(analysis)
- Male
- Middle Aged
- Motor Endplate
(immunology)
- Myasthenia Gravis
(immunology)
- Oculomotor Muscles
- Receptors, Cholinergic
(immunology)
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