Investigation of requirements for efficient gene delivery using the HIV-1 based lentiviral transduction system.

Abstract

The specific recognition and selection of the HIV-1 packaging signal psi (Psi) sequence which is mediated by Gag protein is believed to be pivotal for selective viral genomic RNA packaging and has been a basis for the development of HIV-based transgene delivery systems. However, the requirement of the psi sequence has been questioned recently by a report postulating that the psi element is not absolutely required for transgene transduction. Here, we used a four-plasmid transgene delivery system and analyzed the results by HIV p24 antigen assay, MT4 infection assay, HT1080 colony assay, and reverse transcription-PCR (RT-PCR). The results clearly demonstrate that the psi sequence must be present for efficient transgene encapsidation and transduction.

• Authors: Seon Hee Kim, Soo In Jang, Chul Yong Park, Ji Chang You
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 383 Issue 2 Pg. 192-7 (May 29 2009) ISSN: 1090-2104 [Electronic] United States
• PMID: 19345677 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• enhanced green fluorescent protein
• Green Fluorescent Proteins

Topics

• Cell Line
• Genetic Vectors (genetics)
• Green Fluorescent Proteins (genetics)
• HIV-1 (genetics)
• Humans
• Plasmids (genetics)
• Transduction, Genetic (methods)
• Virus Assembly (genetics)