Quantification of human cytomegalovirus DNA by a new capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay in plasma and peripheral blood mononuclear cells of bone marrow transplant recipients.

Abstract

OBJECTIVES:

Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.

PATIENTS AND METHODS:

Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cytomegalovirus load in specimens of peripheral blood mononuclear cells and plasma.

RESULTS:

All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. In plasma, cytomegalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cytomegalovirus DNA were detected in 14 of 26 patients (53.8%).

CONCLUSIONS:

The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cytomegalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.

• Authors: Mazyar Ziyaeyan, Farzaneh Sabahi, Abdolvahab Alborzi, Mani Ramzi, Fereidoun Mahboudi, Bahman Pourabbas, Mohammad Kadivar
• Journal: Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation (Exp Clin Transplant) Vol. 6 Issue 4 Pg. 294-300 (Dec 2008) ISSN: 1304-0855 [Print] Turkey
• PMID: 19338492 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral

Topics

• Adolescent
• Adult
• Biotinylation
• Bone Marrow Transplantation (adverse effects)
• Child
• Child, Preschool
• Cytomegalovirus (genetics)
• Cytomegalovirus Infections (diagnosis, etiology, genetics, therapy)
• DNA, Viral (blood)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Leukocytes, Mononuclear (virology)
• Male
• Polymerase Chain Reaction
• Predictive Value of Tests
• Prospective Studies
• Time Factors
• Transplantation, Homologous
• Viral Load
• Young Adult