Abstract |
This study was done to determine whether alpha - phenyl-N-tert-butylnitrone (PBN), a spin-trapping agent possessing significant anti-inflammatory capabilities, could attenuate hyperoxia-induced lung injury, and if so, whether this protective effect is mediated by the down-modulation of inflammation in neonatal rats. Newborn Sprague-Dawley rat pups were subjected to 14 days of hyperoxia (> 90% oxygen) within 10 hours after birth. PBN treatment, given 100 mg/kg intraperitoneally daily throughout the experiment, significantly attenuated hyperoxia-induced lung pathology, such as decreased radial alveolar count, increased mean linear intercept, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling-positive cells. Hyperoxia-induced activation of nicotinamide adenine dinucleotide phosphate oxidase that is responsible for superoxide anion production, as evidenced by up-regulation and membrane translocation of p67phox, and the inflammatory responses, such as increased mRNA expression of tumor necrosis factor-alpha, interleukin-6, and transforming growth factor-beta, were also significantly attenuated with PBN treatment. In summary, a spin-trapping agent PBN significantly attenuated hyperoxia-induced lung injury by down-regulating the inflammatory responses in neonatal rats.
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Authors | Yun Sil Chang, Yu Jin Kim, Hye Soo Yoo, Dong Kyung Sung, Soo Yoon Kim, Saem Kang, Won Soon Park |
Journal | Experimental lung research
(Exp Lung Res)
Vol. 35
Issue 3
Pg. 234-49
(Apr 2009)
ISSN: 1521-0499 [Electronic] England |
PMID | 19337906
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclic N-Oxides
- Cytokines
- Free Radical Scavengers
- Phosphoproteins
- RNA, Messenger
- neutrophil cytosol factor 67K
- phenyl-N-tert-butylnitrone
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Topics |
- Animals
- Animals, Newborn
- Cyclic N-Oxides
(pharmacology, therapeutic use)
- Cytokines
(genetics)
- Free Radical Scavengers
(pharmacology, therapeutic use)
- Gene Expression Regulation
- Hyperoxia
(pathology)
- Inflammation
(drug therapy)
- Lung Injury
(drug therapy, etiology, pathology)
- Phosphoproteins
(genetics)
- RNA, Messenger
(analysis)
- Rats
- Rats, Sprague-Dawley
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