Abstract | OBJECTIVES: The aim of this study was to assess the sensitivity, specificity and time to results of mycobacterial growth indicator tube (MGIT) 960, microscopic observation drug susceptibility ( MODS) assay and nitrate reductase assay (NRA) compared with the gold standard agar proportion method (PM), and to determine whether there is cross-resistance between older-generation fluoroquinolones and moxifloxacin. METHODS: RESULTS: Of the 797 M. tuberculosis isolates, 19 (2.4%) were ofloxacin-resistant by PM. MGIT 960 had 100% sensitivity (95% CI, 83%-100%) and specificity (95% CI, 99.5%-100%). Of the 797 isolates, 239 were from 2005 to 2006 and 6 of these (2.5%) were resistant by PM. MODS had 100% sensitivity (95% CI, 61%-100%) and specificity (95% CI, 98%-100%). NRA had 100% sensitivity (95% CI, 61%-100%) and 98.7% specificity (95% CI, 96%-99.6%). The median time to results was shorter using MGIT 960 (8 days), MODS (6 days) or NRA (9 days) compared with PM (21 days) (P < 0.001). All 19 ofloxacin-resistant isolates were resistant to ciprofloxacin, levofloxacin and moxifloxacin by PM. CONCLUSIONS:
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Authors | Rose A Devasia, Amondrea Blackman, Carolyn May, Svetlana Eden, Teresa Smith, Nancy Hooper, Fernanda Maruri, Charles Stratton, Ayumi Shintani, Timothy R Sterling |
Journal | The Journal of antimicrobial chemotherapy
(J Antimicrob Chemother)
Vol. 63
Issue 6
Pg. 1173-8
(Jun 2009)
ISSN: 1460-2091 [Electronic] England |
PMID | 19329799
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Anti-Bacterial Agents
- Fluoroquinolones
- Nitrate Reductase
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Drug Resistance, Bacterial
- Fluoroquinolones
(pharmacology)
- Humans
- Microbial Sensitivity Tests
(methods)
- Microscopy
- Mycobacterium tuberculosis
(cytology, drug effects, growth & development)
- Nitrate Reductase
(metabolism)
- Sensitivity and Specificity
- Time Factors
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